Detailed information of TA system TA185976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 28322..29058
• Replicon: plasmid pE25a
• Accession: NZ_CP067262
• Organism: Klebsiella variicola strain E25

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JJB14_RS26770
• Protein ID: WP_016240380.1
• Coordinates: 28576..29058 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: JJB14_RS26765
• Protein ID: WP_003026799.1
• Coordinates: 28322..28588 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB14_RS26725 (JJB14_26715)	24428..25201	-	774	WP_094833285.1	hypothetical protein	-
JJB14_RS26730 (JJB14_26720)	25214..25714	-	501	WP_116967875.1	HEPN family nuclease	-
JJB14_RS26735 (JJB14_26725)	25989..26237	+	249	WP_094833283.1	hypothetical protein	-
JJB14_RS26740 (JJB14_26730)	26234..26806	+	573	WP_094833282.1	hypothetical protein	-
JJB14_RS26745 (JJB14_26735)	26837..27331	+	495	WP_094833281.1	DNA-binding protein	-
JJB14_RS26750 (JJB14_26740)	27381..27584	+	204	WP_012540085.1	HHA domain-containing protein	-
JJB14_RS26755 (JJB14_26745)	27598..27828	+	231	WP_086071052.1	hypothetical protein	-
JJB14_RS26760 (JJB14_26750)	27892..28146	+	255	WP_208777965.1	hypothetical protein	-
JJB14_RS26765 (JJB14_26755)	28322..28588	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
JJB14_RS26770 (JJB14_26760)	28576..29058	+	483	WP_016240380.1	GNAT family N-acetyltransferase	Toxin
JJB14_RS26775 (JJB14_26765)	29272..30672	+	1401	WP_153669771.1	ISNCY family transposase	-
JJB14_RS26780 (JJB14_26770)	30860..31279	+	420	WP_012540023.1	ASCH domain-containing protein	-
JJB14_RS26785 (JJB14_26775)	31861..32862	+	1002	WP_040107896.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98282	98282
	-	flank	IS/Tn	-	-	29272..30672	1400

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17323.95 Da
• Isoelectric Point: 9.5822

>T185976 WP_016240380.1 NZ_CP067262:28576-29058 [Klebsiella variicola]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T185976 NZ_CP089097:c4737512-4737228 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT185976 WP_003026799.1 NZ_CP067262:28322-28588 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT185976 NZ_CP089097:c4737744-4737502 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66