Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2741370..2742958 Replicon chromosome
Accession NZ_CP067261
Organism Klebsiella variicola strain E25

Toxin (Protein)


Gene name hipA Uniprot ID A0A1F2MCM0
Locus tag JJB14_RS13430 Protein ID WP_008804919.1
Coordinates 2741370..2742692 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A1F2MCN9
Locus tag JJB14_RS13435 Protein ID WP_008804920.1
Coordinates 2742692..2742958 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB14_RS13415 (JJB14_13415) 2736435..2738936 + 2502 WP_208777904.1 fimbria/pilus outer membrane usher protein -
JJB14_RS13420 (JJB14_13420) 2738924..2739928 + 1005 WP_208777906.1 fimbrial protein -
JJB14_RS13425 (JJB14_13425) 2740061..2741353 + 1293 WP_012968243.1 glycoside hydrolase family 10 protein -
JJB14_RS13430 (JJB14_13430) 2741370..2742692 - 1323 WP_008804919.1 type II toxin-antitoxin system HipA family toxin Toxin
JJB14_RS13435 (JJB14_13435) 2742692..2742958 - 267 WP_008804920.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
JJB14_RS13440 (JJB14_13440) 2743224..2743550 - 327 WP_008804921.1 hydroxyisourate hydrolase -
JJB14_RS13445 (JJB14_13445) 2743547..2744047 - 501 WP_008804922.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
JJB14_RS13450 (JJB14_13450) 2744025..2745404 - 1380 WP_012968246.1 nucleobase:cation symporter-2 family protein -
JJB14_RS13455 (JJB14_13455) 2745750..2746904 + 1155 WP_032735567.1 FAD-dependent urate hydroxylase HpxO -
JJB14_RS13460 (JJB14_13460) 2746885..2747808 - 924 WP_023297602.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(13-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 48911.26 Da        Isoelectric Point: 7.9109

>T185969 WP_008804919.1 NZ_CP067261:c2742692-2741370 [Klebsiella variicola]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGGHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMAFLLGSSEALKDRADFMKFMVFQWLVGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPGEQMLAILQEFAGHVP
QAIESARRTLPADFSTHVWRAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T185969 NZ_CP089097:769562-770047 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9936.30 Da        Isoelectric Point: 8.5912

>AT185969 WP_008804920.1 NZ_CP067261:c2742958-2742692 [Klebsiella variicola]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKATETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT185969 NZ_CP089097:769272-769565 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2MCM0

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MCN9

References