Detailed information of TA system TA185956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 326402..326988
• Replicon: chromosome
• Accession: NZ_CP067261
• Organism: Klebsiella variicola strain E25

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A2N4YVJ0
• Locus tag: JJB14_RS01490
• Protein ID: WP_032733892.1
• Coordinates: 326620..326988 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: JJB14_RS01485
• Protein ID: WP_004174006.1
• Coordinates: 326402..326623 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB14_RS01465 (JJB14_01465)	322560..323486	+	927	WP_012540389.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
JJB14_RS01470 (JJB14_01470)	323483..324760	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
JJB14_RS01475 (JJB14_01475)	324757..325524	+	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
JJB14_RS01480 (JJB14_01480)	325526..326239	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
JJB14_RS01485 (JJB14_01485)	326402..326623	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JJB14_RS01490 (JJB14_01490)	326620..326988	+	369	WP_032733892.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JJB14_RS01495 (JJB14_01495)	327280..328596	+	1317	WP_008806974.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
JJB14_RS01500 (JJB14_01500)	328703..329590	+	888	WP_012967120.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
JJB14_RS01505 (JJB14_01505)	329587..330432	+	846	WP_008806976.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
JJB14_RS01510 (JJB14_01510)	330434..331504	+	1071	WP_008806977.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	323483..332241	8758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (8-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13610.96 Da
• Isoelectric Point: 7.3191

>T185956 WP_032733892.1 NZ_CP067261:326620-326988 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPDVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T185956 NZ_CP089092:1444471-1444737 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT185956 WP_004174006.1 NZ_CP067261:326402-326623 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT185956 NZ_CP089092:1444208-1444471 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A2N4YVJ0

Antitoxin

• AlphaFold DB: A0A5E5YJY7