Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 914664..915205 Replicon chromosome
Accession NZ_CP067250
Organism Escherichia coli strain ESBL_DR28

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag JJB16_RS04270 Protein ID WP_000615983.1
Coordinates 914664..914942 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag JJB16_RS04275 Protein ID WP_000729703.1
Coordinates 914945..915205 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJB16_RS04255 (912246) 912246..912824 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
JJB16_RS04260 (913030) 913030..913797 + 768 WP_000333380.1 class II glutamine amidotransferase -
JJB16_RS04265 (913768) 913768..914508 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
JJB16_RS04270 (914664) 914664..914942 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JJB16_RS04275 (914945) 914945..915205 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JJB16_RS04280 (915415) 915415..916164 + 750 WP_000056849.1 C40 family peptidase -
JJB16_RS04285 (916340) 916340..916837 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
JJB16_RS04290 (917061) 917061..918773 - 1713 Protein_842 flagellar biosynthesis protein FlhA -
JJB16_RS04295 (918745) 918745..919530 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 906945..940429 33484


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T185906 WP_000615983.1 NZ_CP067250:c914942-914664 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T185906 NZ_CP089061:c3048989-3048708 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT185906 WP_000729703.1 NZ_CP067250:c915205-914945 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT185906 NZ_CP089061:c3049213-3048986 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References