Detailed information of TA system TA185804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4053962..4054600
• Replicon: chromosome
• Accession: NZ_CP067232
• Organism: Escherichia coli strain SBF45

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: JJB20_RS19235
• Protein ID: WP_000813794.1
• Coordinates: 4054424..4054600 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JJB20_RS19230
• Protein ID: WP_001270286.1
• Coordinates: 4053962..4054378 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB20_RS19210 (4049114)	4049114..4050055	-	942	WP_001251313.1	ABC transporter permease	-
JJB20_RS19215 (4050056)	4050056..4051069	-	1014	WP_001542893.1	ABC transporter ATP-binding protein	-
JJB20_RS19220 (4051087)	4051087..4052232	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
JJB20_RS19225 (4052477)	4052477..4053883	-	1407	WP_000760663.1	PLP-dependent aminotransferase family protein	-
JJB20_RS19230 (4053962)	4053962..4054378	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JJB20_RS19235 (4054424)	4054424..4054600	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JJB20_RS19240 (4054822)	4054822..4055052	+	231	WP_000494244.1	YncJ family protein	-
JJB20_RS19245 (4055144)	4055144..4057105	-	1962	WP_001442195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JJB20_RS19250 (4057178)	4057178..4057714	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
JJB20_RS19255 (4057806)	4057806..4058981	+	1176	WP_001583501.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4059021..4060169	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T185804 WP_000813794.1 NZ_CP067232:c4054600-4054424 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T185804 NZ_CP089031:c4351897-4351499 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT185804 WP_001270286.1 NZ_CP067232:c4054378-4053962 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT185804 NZ_CP089031:c4352193-4351900 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• PDB: 6HPB