Detailed information of TA system TA185730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4177703..4178457
• Replicon: chromosome
• Accession: NZ_CP067091
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain ER3625

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: JJB80_RS20275
• Protein ID: WP_000558166.1
• Coordinates: 4178146..4178457 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JJB80_RS20270
• Protein ID: WP_001259011.1
• Coordinates: 4177703..4178149 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JJB80_RS20240 (4172879)	4172879..4173775	+	897	WP_001520529.1	sugar kinase	-
JJB80_RS20245 (4173809)	4173809..4174612	+	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
JJB80_RS20250 (4174803)	4174803..4175402	+	600	WP_000965695.1	glucose-1-phosphatase	-
JJB80_RS20255 (4175396)	4175396..4176268	+	873	WP_000921427.1	virulence factor BrkB family protein	-
JJB80_RS20260 (4176265)	4176265..4176702	+	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
JJB80_RS20265 (4176747)	4176747..4177688	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
JJB80_RS20270 (4177703)	4177703..4178149	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
JJB80_RS20275 (4178146)	4178146..4178457	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
JJB80_RS20280 (4178543)	4178543..4179472	-	930	WP_001127703.1	alpha/beta hydrolase	-
JJB80_RS20285 (4179690)	4179690..4180001	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
JJB80_RS20290 (4180002)	4180002..4180292	+	291	WP_000362050.1	DNA-binding transcriptional regulator	-
JJB80_RS20295 (4180339)	4180339..4181268	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
JJB80_RS20300 (4181265)	4181265..4181900	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
JJB80_RS20305 (4181897)	4181897..4182799	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T185730 WP_000558166.1 NZ_CP067091:c4178457-4178146 [Salmonella enterica subsp. enterica serovar Typhimurium]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T185730 NZ_CP089012:c2500317-2498995 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTACCGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACTCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT185730 WP_001259011.1 NZ_CP067091:c4178149-4177703 [Salmonella enterica subsp. enterica serovar Typhimurium]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT185730 NZ_CP089012:c2500583-2500317 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90