Detailed information of TA system TA185684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2797946..2799549
• Replicon: chromosome
• Accession: NZ_CP067066
• Organism: Raoultella sp. XY-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JGY86_RS13295
• Protein ID: WP_064367563.1
• Coordinates: 2797946..2799277 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A808YAX5
• Locus tag: JGY86_RS13300
• Protein ID: WP_032717448.1
• Coordinates: 2799277..2799549 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGY86_RS13280	2793820..2794734	-	915	WP_015584333.1	branched-chain amino acid ABC transporter permease	-
JGY86_RS13285	2794834..2795892	-	1059	WP_162180097.1	branched-chain amino acid ABC transporter substrate-binding protein	-
JGY86_RS13290	2796598..2798013	+	1416	WP_099144431.1	glycoside hydrolase family 10 protein	-
JGY86_RS13295	2797946..2799277	-	1332	WP_064367563.1	type II toxin-antitoxin system HipA family toxin	Toxin
JGY86_RS13300	2799277..2799549	-	273	WP_032717448.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JGY86_RS13305	2799752..2800306	-	555	WP_004861926.1	RNA polymerase sigma factor	-
JGY86_RS13310	2800312..2800737	-	426	WP_032724724.1	hypothetical protein	-
JGY86_RS13315	2800870..2801823	-	954	WP_004861921.1	hypothetical protein	-
JGY86_RS13320	2802037..2802669	-	633	WP_048023555.1	class I SAM-dependent methyltransferase	-
JGY86_RS13325	2802689..2803930	-	1242	WP_015584340.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2673912..2901545	227633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49313.63 Da
• Isoelectric Point: 6.7010

>T185684 WP_064367563.1 NZ_CP067066:c2799277-2797946 [Raoultella sp. XY-1]
MATLVTWMNNERVGELIKQANGAHLFRYEEAWMRNPRARPLSLSLPLQYGNITDEAVFNYFDNLLPDSPRVRDRIVKRYQ
ARSKQPFDLLAEIGRDSVGAVTLLPPGENAPRGALSWEPLDNHALDALLTAYQSDIPLGMVNEHDDFRISVAGAQEKTAL
LKIGDQWCIPTGTTPTTHIIKLPIGEIKQPDAVLDLSESVDNEYLCLALARALGFAVPPASIIQTASIRALAVERFDRRW
AQEKTVLLRLPQEDLCQAFGIPSSIKYESDGGPGIRAIMAFLVGSSEALEDRYNFMKFMVFQWLIGATDGHAKNFSIFLQ
PGGSYRLTPFYDIISAFPLLGGKGLHLSDLKLSMSLKASKGRKTEIQTLYPRHFLATAKEVGFAQQQMLEILRYFVDNVP
RAVEAVKGTLPPDFSQPVYEAITDRLLWVHSRLQNAMAASPSG

Nucleotide

• Length: 1332 bp

>T185684 NZ_CP088991:c5396079-5395696 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10365.71 Da
• Isoelectric Point: 5.7971

>AT185684 WP_032717448.1 NZ_CP067066:c2799549-2799277 [Raoultella sp. XY-1]
MSPSPTIYSPVQLANYLKLVRQKNQWTQSELAQRMGIKQATVSNFENHPEKTTLTTVFKILQSLELSMAICEKNAPDTDD
NRDNPRDLEW

Nucleotide

• Length: 273 bp

>AT185684 NZ_CP088991:c5396321-5396079 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A808YAX5