Detailed information of TA system TA185601

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 354849..355329
• Replicon: chromosome
• Accession: NZ_CP067032
• Organism: Agrobacterium fabrum strain EHA105

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A9CK22
• Locus tag: EML540_RS01765
• Protein ID: WP_010971059.1
• Coordinates: 354849..355118 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A9CK21
• Locus tag: EML540_RS01770
• Protein ID: WP_010971060.1
• Coordinates: 355102..355329 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EML540_RS01735	349900..350676	-	777	WP_010971054.1	L,D-transpeptidase	-
EML540_RS01740	350915..351544	+	630	WP_010971055.1	DNA-3-methyladenine glycosylase I	-
EML540_RS01745	351541..351984	+	444	WP_010972617.1	hypothetical protein	-
EML540_RS01750	352010..353053	-	1044	WP_035256280.1	YeiH family protein	-
EML540_RS01755	353135..354052	+	918	WP_010971057.1	LysR family transcriptional regulator	-
EML540_RS01760	354078..354842	-	765	WP_010971058.1	hypothetical protein	-
EML540_RS01765	354849..355118	-	270	WP_010971059.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EML540_RS01770	355102..355329	-	228	WP_010971060.1	ribbon-helix-helix protein, CopG family	Antitoxin
EML540_RS01775	355486..357009	+	1524	WP_010971061.1	histidine--tRNA ligase	-
EML540_RS01780	357014..357382	+	369	WP_010971062.1	VOC family protein	-
EML540_RS01785	357399..358526	+	1128	WP_010971063.1	ATP phosphoribosyltransferase regulatory subunit	-
EML540_RS01790	358523..359215	+	693	WP_010971064.1	ATP phosphoribosyltransferase	-
EML540_RS01795	359280..359726	-	447	WP_006313161.1	DoxX family protein	-
EML540_RS01800	359882..360316	-	435	WP_010971066.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10746.46 Da
• Isoelectric Point: 9.9414

>T185601 WP_010971059.1 NZ_CP067032:c355118-354849 [Agrobacterium fabrum]
VIWTIEYHTLVQKEMRKINPEVRRRIRSFLHERLAALDDPRQIGATLQGSELGNFWRYRVGDYRIICDIQDQKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T185601 NZ_CP088935:c3399798-3399475 [Thauera sp. WB-2]
ATGGTCTTCGTAGAACTCCCTATCTTCAGTCGCTGCGCGCAGGCACTGTTCTCGGACGCACAGCTTCGGGACTTGCAGCA
GGTCTTGCTCGAGAACCCGTCAGTGGGAGATCTGATTCCCGGTGGTCGAGGGCTCCGCAAGTTTCGTGTAGCACTGCTGG
GGCGTGGTAAGCGGGGCGGTGCGCGTGTGATTTATTACCACTGGGTCAGCCGCGAGCAGTGCTACCTGATCTACGCCTAC
GCCAAGAATGCCAGGGCGGACCTGACCAAGGATCAGCTGAGGCGTCTGGCTGAGGTAATCGAAACCGAGGTGAGCAATGG
ATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8937.97 Da
• Isoelectric Point: 4.7254

>AT185601 WP_010971060.1 NZ_CP067032:c355329-355102 [Agrobacterium fabrum]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRIQRGESKIISAEEFWRDLDN

Nucleotide

• Length: 228 bp

>AT185601 NZ_CP088935:c3399482-3399192 [Thauera sp. WB-2]
ATGGATGACAAGCACTTTGAAGAACTGCTGGAAAGCGTTCGTGACATGGGGCGCCACATGCGTGGAGAGCCAGTCGCCGG
CGTTGTCGCGCGAGAGCTGCCCGAGCCAGACGTCAAGGCCATTCGCGAGCGCACGGGGCTGAGCCAGACCCGTTTCGCCT
ATCTCATCGGTGTCAAACCCAAGACGCTGCAGAACTGGGAGCAGCGCCGTGTTCGCCCCGCCGGTCCAGCGCGTGCGCTC
CTCAAGATCGTCGAAGCCAACCCCGCCGCGCTATCGGTGCTTCACCGCTGA

Structures

Toxin

• AlphaFold DB: A9CK22

Antitoxin

• AlphaFold DB: A9CK21