Detailed information of TA system TA185454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3605873..3606651
• Replicon: chromosome
• Accession: NZ_CP066925
• Organism: Xanthomonas campestris strain CFBP5824

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: JH314_RS16025
• Protein ID: WP_223646366.1
• Coordinates: 3606160..3606651 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q8P668
• Locus tag: JH314_RS16020
• Protein ID: WP_011038219.1
• Coordinates: 3605873..3606163 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JH314_RS15995 (JH314_15945)	3601273..3601722	-	450	WP_274339073.1	pilin	-
JH314_RS16000 (JH314_15950)	3602204..3603361	+	1158	WP_274340306.1	type II secretion system F family protein	-
JH314_RS16005 (JH314_15955)	3603368..3604231	+	864	WP_040940797.1	A24 family peptidase	-
JH314_RS16010 (JH314_15960)	3604245..3604868	+	624	WP_076038488.1	dephospho-CoA kinase	-
JH314_RS16015 (JH314_15965)	3605397..3605798	+	402	WP_274339074.1	SymE family type I addiction module toxin	-
JH314_RS16020 (JH314_15970)	3605873..3606163	+	291	WP_011038219.1	DUF1778 domain-containing protein	Antitoxin
JH314_RS16025 (JH314_15975)	3606160..3606651	+	492	WP_223646366.1	GNAT family N-acetyltransferase	Toxin
JH314_RS16030 (JH314_15980)	3606794..3608128	-	1335	WP_274339075.1	HAMP domain-containing sensor histidine kinase	-
JH314_RS16035 (JH314_15985)	3608121..3608798	-	678	WP_003490678.1	response regulator transcription factor	-
JH314_RS16040 (JH314_15990)	3609448..3610323	-	876	WP_070689999.1	30S ribosomal protein S6--L-glutamate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3600767..3641930	41163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17610.25 Da
• Isoelectric Point: 8.1192

>T185454 WP_223646366.1 NZ_CP066925:3606160-3606651 [Xanthomonas campestris]
MTVSAPEPLTAHHRVEPFASGVESLDHWLKRRALKNQATGASRTFVACEDDRVVAYYALASSAVAVDATPGRFRRNMPDP
IPVVVLGRLAVDQSLHGRGFGRALMQDAGKRILHAADTIGIRGLLVHALSADAKAFYERIGFEPSPLDPMVLAVTLEDLK
ASL

Nucleotide

• Length: 492 bp

>T185454 NZ_CP088229:1716345-1716869 [Enterobacter kobei]
ATCGTGGCAAATCTCACGATCGAAATGTTTTCAGAGGGAAGTGATTACGATTTCAGGGGCTTCGATTGTGGTGAACCCTC
GCTCAATGCCTTCCTGACGGAACATCTGGTGCGTCAGCACAAGGGACGGATATTGCGAGGCTACCTGCTTAAAGATCGTG
ACCATCCGCGTGTGCTGGGCTATTACACGTTGTCCGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCTAAGACCCAGCAG
CGTCGGATCCCCTATGTCAACGTACCCAGTGTGACGCTGGGGCGTCTGGCTGTGCATAAAGATCTACAGGGAAAGGAGTG
GGGAACAACGCTCGTCGCACATGCTATGCGCGTTGTGTATCTGGCATCGCAGGCGGTAGGGGTACACGGAATCTTTGTTG
ATGCTCTCAATGAACAGGCAAAACGATTCTATCTGAAGCAGGGTTTTATTCCGCTGACAGGTGAAAACAGCCATTCTCTT
TTCTTCCCCACAAAATCTATCGAACAACTCTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10678.23 Da
• Isoelectric Point: 10.5480

>AT185454 WP_011038219.1 NZ_CP066925:3605873-3606163 [Xanthomonas campestris]
MSTPNTTPGKRETLNLRIKPEERSLIDRAAKARGKNRTDFMLDAARSAAEEALLDQTLITASPDAYAAFLARLDMPPQPN
ARLRKTMQTPAPWEKA

Nucleotide

• Length: 291 bp

>AT185454 NZ_CP088229:1716072-1716341 [Enterobacter kobei]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAGACAATCACCCAGTTTATGGTGAACAGTGCGTCAGAACGCGCTGCTGAAGTCATCGAACAACATCGGCGTC
TCGTATTAAACGAGTCATCATGGAATGCGGTGATGGACGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCAAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: Q8P668