Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 2108..2667 Replicon plasmid pIncI1_STnovel
Accession NZ_CP066838
Organism Escherichia coli strain Ec-FL1-1X

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag JH476_RS25320 Protein ID WP_000421257.1
Coordinates 2392..2667 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A418GAX7
Locus tag JH476_RS25315 Protein ID WP_024186313.1
Coordinates 2108..2392 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JH476_RS25310 (1) 1..1032 + 1032 WP_000907863.1 plasmid replication initiator RepA -
JH476_RS25315 (2108) 2108..2392 + 285 WP_024186313.1 ribbon-helix-helix domain-containing protein Antitoxin
JH476_RS25320 (2392) 2392..2667 + 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JH476_RS25325 (2773) 2773..2976 + 204 Protein_3 hypothetical protein -
JH476_RS25330 (3245) 3245..3511 - 267 WP_000627834.1 hypothetical protein -
JH476_RS25335 (3580) 3580..4005 - 426 WP_001389390.1 hypothetical protein -
JH476_RS25340 (4061) 4061..4663 - 603 WP_000517689.1 ProQ/FINO family protein -
JH476_RS25345 (5017) 5017..6363 + 1347 WP_001132019.1 protein YagA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..95616 95616


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T185167 WP_000421257.1 NZ_CP066838:2392-2667 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T185167 NZ_CP088081:c2564486-2563986 [Ideonella dechloratans]
GTGACGTTGCGCGCACCGGAGCCTCTGGCCGCGCAGCATCGGCTGGAAGGTTTTGATTGCGGCAAGCCCGCGTTGAATGA
CTGGCTGTTGCGCCACGCCCGCCAGGCGCAGGGCAGTGGCTCGGCCAAGACCTTTGTCGTTGCCGAGGACGATGGCCGCG
TGGCGGGCTACTTTAGCCTGACCGTGGGACAAGTCGACACGCTGGAGGCGCCGGAGCGCATTCGCAAGGGGATGGGGCAG
TACCCGTTGCCGGTGGTGATTCTGGCGAGGCTCGCTGTCTCGGTGGTGGACCAGGGGCGAGGGATAGGCTTTGGCCTGCT
GCAGGACGCGATTCGCCGCACGATGCTGATTGCCGAACAGGCCGGCATCCGTGCCATGCTGACCCACCCCATCGATGAAG
AAGCGGCGAGGTTCTACACCCGGTTCGGGTTCATCGCGTCACCGCTGCGCGAACAGCAATTGTTGCTGCTCTTGAAGGAC
GCCCGTCGCTGGGTCCGCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10504.92 Da        Isoelectric Point: 5.6558

>AT185167 WP_024186313.1 NZ_CP066838:2108-2392 [Escherichia coli]
MQMKNNTAQTTKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT185167 NZ_CP088081:c2564776-2564483 [Ideonella dechloratans]
ATGACTGCCGTTGCATCGTCGCCTTCCGCCCGTTCTGCCCGACTTGGGCTGCGCGCTACCCCCGAACAGGAGGTGGTGCT
GCGCCGTGCCGCCGAGGTGGCCCACAAGTCGTTGACCGACTTCATCCTCGACAGCGCGTGCCTGGCCGCCGAGCAGACGC
TGCTGGACCAACGATTGTTCATGGTCTCGGGCAGCCAGTACCAGGCCCTGATGGATCTGCTGGATCGCCCCGAACAAGCC
AACGAGGGGTTGCGTGACCTGTTTGCCCGCAAGGCGCCCTGGGATACGAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A418GAX7

References