Detailed information of TA system TA184924

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 17654..17918
• Replicon: plasmid unnamed1
• Accession: NZ_CP066754
• Organism: Escherichia coli O157:H7 strain ECP19-598

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: JG531_RS27655
• Protein ID: WP_001303307.1
• Coordinates: 17766..17918 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	17654..17711 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG531_RS27640	12893..15184	-	2292	WP_001289282.1	hypothetical protein	-
JG531_RS27645	15177..16247	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
JG531_RS27650	16266..17474	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	17654..17711	-	58	NuclAT_0	-	Antitoxin
-	17654..17711	-	58	NuclAT_0	-	Antitoxin
-	17654..17711	-	58	NuclAT_0	-	Antitoxin
-	17654..17711	-	58	NuclAT_0	-	Antitoxin
JG531_RS27655	17766..17918	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
JG531_RS27660	17990..18241	-	252	WP_001291965.1	hypothetical protein	-
JG531_RS27665	18900..19076	-	177	WP_001054897.1	hypothetical protein	-
JG531_RS27670	19468..19677	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
JG531_RS27675	19749..20399	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
JG531_RS27680	20473..22641	-	2169	WP_171880343.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..89397	89397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T184924 WP_001303307.1 NZ_CP066754:17766-17918 [Escherichia coli O157:H7]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T184924 NZ_CP087843:c2823127-2822855 [Moraxella bovis]
GTGGCTAAACGTAGTTTTGAAATGGGCAATCCCTTTAAAAGGGATTTAAAAAAGCATTATTTGGCATTGATTGGCGAAGC
GTGGACGGAAGTGTCTGATTGCTTGTTAAATGCCAAACCCATACCTCAAAAATACCAAGACCACCCATTGCAAGGCGATT
GGGTGGGGTTTAGAGATTGTCATATCAAGCCTGATTTGGTGCTAATTTATAAAATAAATGATGAAGCGGTGGAATTACAT
CGGCTTGCCACGCATAGCGAGTTGTTTGGTTGA

Antitoxin

Nucleotide

• Length: 58 bp

>AT184924 NZ_CP066754:c17711-17654 [Escherichia coli O157:H7]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin