Detailed information of TA system TA184869

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2944434..2944659
• Replicon: chromosome
• Accession: NZ_CP066748
• Organism: Escherichia coli O157:H7 strain ECP19-2498

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: JG532_RS14870
• Protein ID: WP_000813258.1
• Coordinates: 2944434..2944589 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2944601..2944659 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG532_RS14820	2939437..2939868	-	432	WP_001302123.1	tellurite resistance protein	-
JG532_RS14835	2940319..2941032	-	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
JG532_RS14840	2941168..2941365	-	198	WP_000917763.1	hypothetical protein	-
JG532_RS14845	2941590..2942144	-	555	WP_000640035.1	DUF1133 family protein	-
JG532_RS14850	2942207..2942512	-	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
JG532_RS14855	2942525..2943574	-	1050	WP_001265229.1	DUF968 domain-containing protein	-
JG532_RS14860	2943576..2943848	-	273	WP_000191872.1	hypothetical protein	-
JG532_RS14865	2943970..2944314	-	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
JG532_RS14870	2944434..2944589	-	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	2944601..2944659	+	59	-	-	Antitoxin
JG532_RS14875	2944880..2945437	-	558	WP_000104474.1	DUF551 domain-containing protein	-
JG532_RS14880	2945439..2945657	-	219	WP_000683609.1	DUF4014 family protein	-
JG532_RS14885	2945785..2946096	-	312	WP_001289673.1	hypothetical protein	-
JG532_RS14890	2946089..2946316	-	228	WP_000699809.1	hypothetical protein	-
JG532_RS14895	2946313..2946594	-	282	WP_000603384.1	DNA-binding protein	-
JG532_RS14900	2946627..2947343	-	717	WP_000450627.1	DUF1627 domain-containing protein	-
JG532_RS14905	2947377..2947838	-	462	WP_000139447.1	replication protein	-
JG532_RS14910	2947831..2948874	-	1044	WP_001262402.1	hypothetical protein	-
JG532_RS14915	2948943..2949368	-	426	WP_000693878.1	Rha family transcriptional regulator	-
JG532_RS14920	2949352..2949594	-	243	WP_000747948.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	paa	2900421..2987997	87576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T184869 WP_000813258.1 NZ_CP066748:c2944589-2944434 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T184869 NZ_CP087828:428794-429066 [Moraxella bovis]
GTGGCTAAACGTAGTTTTGAAATGGGCAATCCCTTTAAAAGGGATTTAAAAAAGCATTATTTGGCATTGATTGGCGAAGC
GTGGACGGAAGTGTCTGATTGCTTGTTAAATGCCAAACCCATACCTCAAAAATACCAAGACCACCCATTGCAAGGCGATT
GGGTGGGGTTTAGAGATTGTCATATCAAGCCTGATTTGGTGCTAATTTATAAAATAAATGATGAAGCGGTGGAATTACAT
CGGCTTGCCACGCATAGCGAGTTGTTTGGTTGA

Antitoxin

Nucleotide

• Length: 59 bp

>AT184869 NZ_CP066748:2944601-2944659 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin