Detailed information of TA system TA184588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 3071..3545
• Replicon: plasmid p3_090361
• Accession: NZ_CP066533
• Organism: Klebsiella pneumoniae strain WCHKP090361

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: H3G66_RS29015
• Protein ID: WP_004197762.1
• Coordinates: 3071..3337 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: H3G66_RS29020
• Protein ID: WP_000879771.1
• Coordinates: 3321..3545 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G66_RS29000	858..1237	+	380	Protein_0	hypothetical protein	-
H3G66_RS29005	1263..1808	+	546	WP_023317533.1	hypothetical protein	-
H3G66_RS29010	2210..2907	-	698	Protein_2	IS1 family transposase	-
H3G66_RS29015	3071..3337	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H3G66_RS29020	3321..3545	-	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
H3G66_RS29025	4212..4424	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
H3G66_RS29030	4431..4940	-	510	WP_032433972.1	MbeB family mobilization protein	-
H3G66_RS29035	4915..5679	-	765	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
H3G66_RS29040	5618..5962	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
H3G66_RS29045	5952..6128	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..6373	6373
	-	flank	IS/Tn	-	-	2210..2506	296

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T184588 WP_004197762.1 NZ_CP066533:c3337-3071 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T184588 NZ_CP087691:1463540-1463863 [Xylella taiwanensis]
ATGACTCAACGCGGCGACCTTGTAACGGTTTCCCTGCAAGGCGATTATGGCAAACCTAGACCGGCGTTAATCCTCCAATC
AGACCTGCTGATGGAGCTAGATAGTGTCGTACTGTGTCCGGTCACAAGTGAGCTACGTAATGCGATATTTCGCATCACCG
TTGAACCAAACATAGCCAATGGGCTACGGACGCTCTCGCAAATCATGGTAGACAAAATTTCAACACTGCCGCGTAGCAAA
ATCAGCAAACCTTTTGGACATCTCAATGACGAGAGGATAAAAGCAGTAGAAAAAGCACTGTTGCTCATCGTTGGTATCAT
CTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT184588 WP_000879771.1 NZ_CP066533:c3545-3321 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT184588 NZ_CP087691:1463322-1463543 [Xylella taiwanensis]
ATGGCATTCATTCACGAACCGACTAGCAAAGTAGCTCGCCACCGAAAGCAAATGCGCGCAGCGGGATTACGTCCTGTCCA
GTTCTGGGTACCGGATACGCGCACACCGGAATTCGCCGCTGAAATTCGGAGCCAGTGCAGGAGCCTGAAGAACGACCCGG
AAGAAGCTGACGCTTTGCGCTTCACCGAAGAAGCCATAACCCATATTGAAGGCTGGAAATGA

Structures

Antitoxin

• AlphaFold DB: S1EJF5