Detailed information of TA system TA184524

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 91056..91309
• Replicon: plasmid pKPC2_090329
• Accession: NZ_CP066518
• Organism: Klebsiella pneumoniae strain WCHKP090329

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H3G99_RS27250
• Protein ID: WP_001312851.1
• Coordinates: 91056..91205 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	91250..91309 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3G99_RS27215	86415..86830	-	416	Protein_113	IS1 family transposase	-
H3G99_RS27220	87079..87480	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
H3G99_RS27225	87413..87670	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H3G99_RS27230	87763..88416	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
H3G99_RS27235	89355..90212	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
H3G99_RS27240	90205..90279	-	75	WP_001375168.1	RepA leader peptide Tap	-
H3G99_RS27245	90524..90772	-	249	WP_000083837.1	replication regulatory protein RepA	-
H3G99_RS27250	91056..91205	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	91250..91309	+	60	NuclAT_1	-	Antitoxin
-	91250..91309	+	60	NuclAT_1	-	Antitoxin
-	91250..91309	+	60	NuclAT_1	-	Antitoxin
-	91250..91309	+	60	NuclAT_1	-	Antitoxin
H3G99_RS27255	91510..91740	-	231	WP_001736714.1	hypothetical protein	-
H3G99_RS27260	91904..92503	-	600	WP_032083981.1	hypothetical protein	-
H3G99_RS27265	92889..93089	-	201	WP_015059022.1	hypothetical protein	-
H3G99_RS27270	93221..93781	-	561	WP_000139328.1	fertility inhibition protein FinO	-
H3G99_RS27275	93836..94582	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
H3G99_RS27280	94661..95365	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H3G99_RS27285	95418..95483	+	66	Protein_127	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65	-	1..149181	149181

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T184524 WP_001312851.1 NZ_CP066518:c91205-91056 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T184524 NZ_CP087677:2824032-2824448 [Xylella taiwanensis]
TTGAGCGGATATTTGCTCGATACGAACATCATTAGCGACATGATCCGTAATCCGTTTGGACTAGTTGCGTGTCGCATTGA
AAAGGTCGATCCAAAAGAGGTTTGCACTAGCATCGTTGTCGCTGCTGAGTTGCGGTATGGTTGTGCAAAGAAAGGATCGA
CCAAGTTGCTTGCGAAGGTGAAGCTCATGCTTGAAACAATTCCTGTGCTGCCTTTGGATATTCCTACCGACGCTGAATAT
GGTCGCATTCGTGCAGAGTTGGAGTCAGTAGGACAAACTATCGGCTTAAATGATCTATTGATAGGTACTCATGCTTATGT
ACTGGGGATGACATTGGTAACGGATAATGTTAAAGAGTTTAAGCGTATTCGTGGCCTTAAGATCGAAAATTGGCTGGAAT
GTGCATCCTCGGTTTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT184524 NZ_CP066518:91250-91309 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin