Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 6376..6869 Replicon plasmid unnamed2
Accession NZ_CP066368
Organism Escherichia coli strain AR Bank #0346

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag FNX50_RS23355 Protein ID WP_001230709.1
Coordinates 6376..6630 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag FNX50_RS23360 Protein ID WP_000151456.1
Coordinates 6627..6869 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNX50_RS24045 1783..1973 + 191 Protein_1 hypothetical protein -
FNX50_RS24050 2623..2775 + 153 Protein_2 DUF5431 family protein -
FNX50_RS23325 (FNX50_23320) 2720..2842 + 123 WP_223170659.1 type I toxin-antitoxin system Hok family toxin -
FNX50_RS23330 (FNX50_23325) 2992..3588 + 597 WP_000125165.1 ProQ/FINO family protein -
FNX50_RS23335 (FNX50_23330) 3606..3890 - 285 WP_001162371.1 hypothetical protein -
FNX50_RS23340 (FNX50_23335) 3964..4629 - 666 WP_000532148.1 division plane positioning ATPase MipZ -
FNX50_RS23345 (FNX50_23340) 5218..5733 + 516 WP_001025393.1 J domain-containing protein -
FNX50_RS23350 (FNX50_23345) 5837..6379 + 543 WP_001083904.1 hypothetical protein -
FNX50_RS23355 (FNX50_23350) 6376..6630 + 255 WP_001230709.1 hypothetical protein Toxin
FNX50_RS23360 (FNX50_23355) 6627..6869 + 243 WP_000151456.1 hypothetical protein Antitoxin
FNX50_RS23365 (FNX50_23360) 6994..7338 + 345 Protein_11 WbuC family cupin fold metalloprotein -
FNX50_RS23370 (FNX50_23365) 7385..8260 - 876 WP_015387340.1 extended-spectrum class A beta-lactamase CTX-M-55 -
FNX50_RS23375 (FNX50_23370) 8513..9774 - 1262 Protein_13 IS1380-like element ISEcp1 family transposase -
FNX50_RS23380 (FNX50_23375) 10533..10862 + 330 WP_000855120.1 hypothetical protein -
FNX50_RS23385 (FNX50_23380) 10971..11117 - 147 WP_001356709.1 hypothetical protein -
FNX50_RS23390 (FNX50_23385) 11443..11766 + 324 WP_000427403.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-55 / mcr-1.1 - 1..66038 66038
- flank IS/Tn blaCTX-M-55 - 7385..9774 2389


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T184364 WP_001230709.1 NZ_CP066368:6376-6630 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T184364 NZ_CP087629:715145-715630 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT184364 WP_000151456.1 NZ_CP066368:6627-6869 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT184364 NZ_CP087629:714855-715148 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References