Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 166817..167397 Replicon plasmid pSymA
Accession NZ_CP066361
Organism Sinorhizobium meliloti strain AK76

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag JFX10_RS26355 Protein ID WP_010968151.1
Coordinates 166817..167200 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag JFX10_RS26360 Protein ID WP_013851145.1
Coordinates 167197..167397 (-) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX10_RS26330 162047..162985 + 939 WP_122103135.1 nucleoside hydrolase -
JFX10_RS26335 162982..164508 + 1527 WP_014532023.1 amidohydrolase -
JFX10_RS26340 164699..165022 - 324 WP_003526806.1 DNA-binding transcriptional regulator -
JFX10_RS26345 165012..165370 - 359 Protein_153 type II toxin-antitoxin system RelE/ParE family toxin -
JFX10_RS26350 165927..166663 + 737 Protein_154 RepB plasmid partition -
JFX10_RS26355 166817..167200 - 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
JFX10_RS26360 167197..167397 - 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JFX10_RS26365 167912..168439 + 528 WP_198869060.1 TIGR02444 family protein -
JFX10_RS26370 168630..169403 + 774 WP_014528246.1 UTRA domain-containing protein -
JFX10_RS26375 169495..170871 - 1377 WP_010968148.1 NAD(P)/FAD-dependent oxidoreductase -
JFX10_RS26380 170868..171182 - 315 WP_198869061.1 (2Fe-2S)-binding protein -
JFX10_RS26385 171172..172278 - 1107 WP_010968147.1 FAD-binding oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(3)-IIc htpB / htpB / katA 1..1580177 1580177


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T184329 WP_010968151.1 NZ_CP066361:c167200-166817 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T184329 NZ_CP087618:325461-325829 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT184329 WP_013851145.1 NZ_CP066361:c167397-167197 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT184329 NZ_CP087618:325243-325464 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References