Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 58966..59499 Replicon chromosome
Accession NZ_CP066265
Organism Pseudomonas syringae strain PA-2-9E

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag I9H07_RS00290 Protein ID WP_094188367.1
Coordinates 58966..59184 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag I9H07_RS00295 Protein ID WP_236423336.1
Coordinates 59194..59499 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I9H07_RS00260 (I9H07_00260) 54014..55507 - 1494 WP_058391003.1 YifB family Mg chelatase-like AAA ATPase -
I9H07_RS00265 (I9H07_00265) 55723..56214 - 492 WP_236423339.1 RES family NAD+ phosphorylase -
I9H07_RS00270 (I9H07_00270) 56211..56687 - 477 WP_236423338.1 DUF2384 domain-containing protein -
I9H07_RS00275 (I9H07_00275) 56868..57602 - 735 WP_058391001.1 SDR family oxidoreductase -
I9H07_RS00280 (I9H07_00280) 57828..58745 + 918 WP_058391000.1 AraC family transcriptional regulator -
I9H07_RS00285 58761..58961 - 201 Protein_56 hypothetical protein -
I9H07_RS00290 58966..59184 + 219 WP_094188367.1 proteic killer protein Toxin
I9H07_RS00295 (I9H07_00285) 59194..59499 + 306 WP_236423336.1 HigA family addiction module antitoxin Antitoxin
I9H07_RS00300 (I9H07_00290) 59533..59799 - 267 WP_024672112.1 accessory factor UbiK family protein -
I9H07_RS00305 (I9H07_00295) 60219..60557 + 339 WP_002555808.1 P-II family nitrogen regulator -
I9H07_RS00310 (I9H07_00300) 60592..61929 + 1338 WP_236423335.1 ammonium transporter -
I9H07_RS00315 (I9H07_00305) 62217..62642 + 426 WP_058391016.1 secondary thiamine-phosphate synthase enzyme YjbQ -
I9H07_RS00320 (I9H07_00310) 62740..63069 + 330 WP_024643544.1 transcriptional regulator SutA -
I9H07_RS00325 (I9H07_00315) 63150..63842 - 693 WP_058390999.1 HAD-IA family hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(26-72)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8355.61 Da        Isoelectric Point: 7.8884

>T184255 WP_094188367.1 NZ_CP066265:58966-59184 [Pseudomonas syringae]
VIVSFRCVHTSDLFEHGKTRLWASIKSVAERKLAMLDGDRFGQYSIRINAQFRICFIWGVNGPENVEIIDYH

Download         Length: 219 bp

>T184255 NZ_CP087579:c4845-4429 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11229.03 Da        Isoelectric Point: 8.5239

>AT184255 WP_236423336.1 NZ_CP066265:59194-59499 [Pseudomonas syringae]
MNKNGMRPVHPGEVLREEYLEPMRLTAAALARALGVSTPTVNDIVLQRRGVSADVALRLAACLETSPEFWLNLQLAYDLR
KAEIEKGAQIREQVRSLARCA

Download         Length: 306 bp

>AT184255 NZ_CP087579:c5072-4842 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References