Detailed information of TA system TA184161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3271280..3272272
• Replicon: chromosome
• Accession: NZ_CP066194
• Organism: Bacillus cytotoxicus strain E8.1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A7GPN6
• Locus tag: JC776_RS16750
• Protein ID: WP_012094283.1
• Coordinates: 3271532..3272272 (-)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A7GPN7
• Locus tag: JC776_RS16745
• Protein ID: WP_012094285.1
• Coordinates: 3271280..3271408 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JC776_RS16720 (JC776_16720)	3266876..3267127	-	252	WP_012094290.1	hypothetical protein	-
JC776_RS16725 (JC776_16725)	3267726..3269195	-	1470	WP_087094928.1	aminoacyl-histidine dipeptidase	-
JC776_RS16730 (JC776_16730)	3269401..3270282	+	882	WP_087094927.1	aminoglycoside 6-adenylyltransferase	-
JC776_RS16735 (JC776_16735)	3270323..3270868	+	546	WP_087094926.1	GNAT family N-acetyltransferase	-
JC776_RS16740 (JC776_16740)	3271027..3271203	-	177	WP_087094925.1	stage II sporulation protein SB	-
JC776_RS16745 (JC776_16745)	3271280..3271408	-	129	WP_012094285.1	hypothetical protein	Antitoxin
JC776_RS16750 (JC776_16750)	3271532..3272272	-	741	WP_012094283.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
JC776_RS16755 (JC776_16755)	3272467..3273204	-	738	WP_012094282.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
JC776_RS16760 (JC776_16760)	3273342..3273824	-	483	WP_012094281.1	helix-turn-helix domain-containing protein	-
JC776_RS16765 (JC776_16765)	3274044..3275348	-	1305	WP_087094924.1	DEAD/DEAH box helicase	-
JC776_RS16770 (JC776_16770)	3275543..3275848	+	306	Protein_3246	hypothetical protein	-
JC776_RS16775 (JC776_16775)	3275895..3276797	-	903	WP_012094278.1	BadF/BadG/BcrA/BcrD ATPase family protein	-
JC776_RS16780 (JC776_16780)	3276867..3277132	-	266	Protein_3248	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28628.30 Da
• Isoelectric Point: 6.7302

>T184161 WP_012094283.1 NZ_CP066194:c3272272-3271532 [Bacillus cytotoxicus]
MTVTSVRIGLFFLVIFFLFLIFFYWRNEERYEERKQLIRKTWYGLFITSVTMYFMMKGIDLTLWKHILMVVAMVVFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTLIQNVNPAAFGTMEWETDAEYARSLNAFLDTYAEKIGAK
IVVFVTEEELNTNFRGVRSQFSITVSPESIEQLNEQKAVQIENVGIIPAKIIKDVYIIMDGQKNNLQDRDFENVYNLTIH
HSYFCK

Nucleotide

• Length: 741 bp

>T184161 NZ_CP087392:699273-699548 [Pectobacterium parvum]
ATGGAAATTAAATGGACAAGTAAGGCACTGTCAGATTTAGCTCATTTGTATGAATTTCTGGCTCTGGCTAACAAATCTGC
TGCTGCAAGAGCAGTTCAAGAATTGACTCAGGCTCCGGCCATCCTGTTAACCAATTCACGCCTTGGGGAGCAATTATTCC
AGTTCGAGCCCCGGGAAATCAGACGATTTCTGGTTGGAGAGTATGAGGTCCGCCATGAAATTGAGAAGTCCGTCATCTAC
GTGCTGCGCATCTGGCATGCCAGAGAGGATCGCTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4984.43 Da
• Isoelectric Point: 3.9944

>AT184161 WP_012094285.1 NZ_CP066194:c3271408-3271280 [Bacillus cytotoxicus]
VLKEYSNDFFKDEAEDTTDFSLIKGDTMQQVEELEEELKRRG

Nucleotide

• Length: 129 bp

>AT184161 NZ_CP087392:698995-699273 [Pectobacterium parvum]
ATGAAGAACGATACGCAGGTAGTAACTAGGGTGCTGACAGCTCATGTGCCATTGCCCATAGCTGATAAGGTTGATCAAAT
TGCAGCCCGACTTGAACGTTCACGAGGCTGGATCATGAAGCAGGCGCTTTCCGCATGGATAGCCCAGGAAGAGGAGCGAG
ACCGCCTGACCCAGGAAGCGCTGGCAGATGTGGATGCGGGACGGGTTATCGATCACCAGGCCGTGCAGGCATGGGCTGAC
AGCCTTGGAACCGACAAACCATTGCCGGTTCCGCGTTGA

Structures

Toxin

• AlphaFold DB: A7GPN6

Antitoxin

• AlphaFold DB: A7GPN7