Detailed information of TA system TA184127

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3784541..3785195
• Replicon: chromosome
• Accession: NZ_CP066169
• Organism: Pseudomonas simiae strain K-Hf-L9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ICJ33_RS17110
• Protein ID: WP_042572419.1
• Coordinates: 3784541..3784891 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: C3JY95
• Locus tag: ICJ33_RS17115
• Protein ID: WP_010209571.1
• Coordinates: 3784881..3785195 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICJ33_RS17100	3781299..3782831	+	1533	WP_010209575.1	NADH-quinone oxidoreductase subunit M	-
ICJ33_RS17105	3782839..3784302	+	1464	WP_012724956.1	NADH-quinone oxidoreductase subunit NuoN	-
ICJ33_RS17110	3784541..3784891	+	351	WP_042572419.1	toxin	Toxin
ICJ33_RS17115	3784881..3785195	+	315	WP_010209571.1	XRE family transcriptional regulator	Antitoxin
ICJ33_RS17120	3785257..3786009	-	753	WP_010209570.1	outer membrane beta-barrel protein	-
ICJ33_RS17125	3786143..3786970	-	828	WP_198581014.1	arylamine N-acetyltransferase	-
ICJ33_RS17130	3787091..3787327	+	237	WP_010209566.1	hypothetical protein	-
ICJ33_RS17135	3787359..3787559	-	201	WP_010209565.1	hypothetical protein	-
ICJ33_RS17140	3787574..3787750	-	177	WP_010209563.1	hypothetical protein	-
ICJ33_RS17145	3787864..3788385	+	522	WP_010209560.1	DUF3087 domain-containing protein	-
ICJ33_RS17150	3788427..3789557	-	1131	WP_069672538.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13730.57 Da
• Isoelectric Point: 8.5175

>T184127 WP_042572419.1 NZ_CP066169:3784541-3784891 [Pseudomonas simiae]
MDALFIELPAFERHRKDYLSDELFHGFQQALMKNPEAGDVIEGTGGLRKVSFVDERRNKGKRGGLRVIYYWWSDGTQFWL
FTLYGKHEQDDLTPQHKKALKHMLDREIKARTHHET

Nucleotide

• Length: 351 bp

>T184127 NZ_CP087377:3470430-3470816 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11741.51 Da
• Isoelectric Point: 10.4449

>AT184127 WP_010209571.1 NZ_CP066169:3784881-3785195 [Pseudomonas simiae]
MKREIFSELVEGFDALANERQGKVTLRTHKVHLNNLEPLTAEEVVAVRQQLNLSRAVFAMYLRTNTRTLENWEQGRAKPN
AQAATLIRLVARFPETVAQLAALG

Nucleotide

• Length: 315 bp

>AT184127 NZ_CP087377:3470806-3471135 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2N5F9B6