Detailed information of TA system TA184126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1923969..1924573
• Replicon: chromosome
• Accession: NZ_CP066169
• Organism: Pseudomonas simiae strain K-Hf-L9

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1N7UCS2
• Locus tag: ICJ33_RS08880
• Protein ID: WP_010211777.1
• Coordinates: 1923969..1924157 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1N7UII5
• Locus tag: ICJ33_RS08885
• Protein ID: WP_010211776.1
• Coordinates: 1924169..1924573 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICJ33_RS08850	1919642..1920178	+	537	WP_205517970.1	DsbE family thiol:disulfide interchange protein	-
ICJ33_RS08855	1920175..1920648	+	474	WP_010211783.1	cytochrome c-type biogenesis protein CcmH	-
ICJ33_RS08860	1920641..1921828	+	1188	WP_198581521.1	c-type cytochrome biogenesis protein CcmI	-
ICJ33_RS08865	1921845..1922240	+	396	WP_010211781.1	hypothetical protein	-
ICJ33_RS08870	1922576..1923148	+	573	WP_042570797.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
ICJ33_RS08875	1923173..1923649	-	477	WP_045792371.1	GNAT family N-acetyltransferase	-
ICJ33_RS08880	1923969..1924157	+	189	WP_010211777.1	type II toxin-antitoxin system HicA family toxin	Toxin
ICJ33_RS08885	1924169..1924573	+	405	WP_010211776.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ICJ33_RS08890	1924879..1926435	+	1557	WP_044286877.1	M10 family metallopeptidase C-terminal domain-containing protein	-
ICJ33_RS08895	1926445..1927641	-	1197	WP_021492219.1	MFS transporter	-
ICJ33_RS08900	1927818..1928432	+	615	WP_021492218.1	hypothetical protein	-
ICJ33_RS08905	1928562..1929566	-	1005	WP_010211770.1	sulfate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7161.38 Da
• Isoelectric Point: 11.0014

>T184126 WP_010211777.1 NZ_CP066169:1923969-1924157 [Pseudomonas simiae]
VQSRLLIKELQEAGWVLDRVAGSHHIFTHRYNPYTIPVPHPKKDLPLGTVKSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T184126 NZ_CP087377:3215327-3215821 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14683.64 Da
• Isoelectric Point: 4.6000

>AT184126 WP_010211776.1 NZ_CP066169:1924169-1924573 [Pseudomonas simiae]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHIMLQEIAAEGGPIPLPTSVANHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT184126 NZ_CP087377:3215037-3215330 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A1N7UCS2

Antitoxin

• AlphaFold DB: A0A1N7UII5