Detailed information of TA system TA184073

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3092855..3093689
• Replicon: chromosome
• Accession: NZ_CP066144
• Organism: Escherichia coli strain ZLWT

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: ICI43_RS14935
• Protein ID: WP_000854690.1
• Coordinates: 3092855..3093232 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ICI43_RS14940
• Protein ID: WP_001546171.1
• Coordinates: 3093321..3093689 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI43_RS14900	3088446..3089000	-	555	WP_001001909.1	molecular chaperone YcdY	-
ICI43_RS14905	3089024..3089761	-	738	WP_000283664.1	zinc-binding phosphatase	-
ICI43_RS14910	3089816..3090754	-	939	WP_000351287.1	glyoxylate/hydroxypyruvate reductase GhrA	-
ICI43_RS14920	3091241..3092068	-	828	WP_001304477.1	DUF4942 domain-containing protein	-
ICI43_RS14925	3092153..3092350	-	198	WP_000772027.1	DUF957 domain-containing protein	-
ICI43_RS14930	3092370..3092858	-	489	WP_001546173.1	hypothetical protein	-
ICI43_RS14935	3092855..3093232	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
ICI43_RS14940	3093321..3093689	-	369	WP_001546171.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ICI43_RS14945	3093739..3094383	-	645	WP_000094915.1	hypothetical protein	-
ICI43_RS14950	3094402..3094623	-	222	WP_000692329.1	DUF987 domain-containing protein	-
ICI43_RS14955	3094686..3095162	-	477	WP_001537421.1	RadC family protein	-
ICI43_RS14960	3095178..3095651	-	474	WP_000855076.1	antirestriction protein	-
ICI43_RS14965	3095914..3096735	-	822	WP_001234710.1	DUF945 domain-containing protein	-
ICI43_RS14970	3096816..3097027	+	212	Protein_2940	hypothetical protein	-
ICI43_RS14975	3097146..3097601	-	456	WP_000581502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T184073 WP_000854690.1 NZ_CP066144:c3093232-3092855 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T184073 NZ_CP087325:c419511-419110 [Acinetobacter baumannii]
ATGTTGAGATATATGTTAGATACCAATATCGTGATCTATACCATCAAAAATAAACCGCCTGAAGTTAAAGTGCGATTTAA
TGCTGTTGCCACTCAGTTATGTGTCAGTAGTATCACTGTGGCAGAACTTATTTATGGTGCAGAAAATAGTCAAAACCCTA
TAAAAAATTTACGCGTTGTCGAAGATTTTTTATCTCGATTACAGATTTTAGATTATACCCCTGAAGCAGCAGCTCAATAT
GGCATGATCAAAGCAGATTTACGTCGTAAAGGCACGCTTATTAGTGATAATGACACGCATATTGCAGCTCATGCTCGTAG
TCATGGTCTTATTTTAGTCACCAACAATACTAAAGAATTTCAAAGAGTCGAAGCTTTACAGTTAGAGAATTGGGTTGAGT
GA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13745.37 Da
• Isoelectric Point: 4.6220

>AT184073 WP_001546171.1 NZ_CP066144:c3093689-3093321 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT184073 NZ_CP087325:c419738-419511 [Acinetobacter baumannii]
ATGATTACATCTGTATTTACAAATAATCGCTCCCAAGCTGTTCGCTTACCAGCTATTTTTCGCTTTCCTAAAGAAACACA
AAAAGTCTTTGTAAGGGTCGTTGGTGTAGAACGCGTCATTTGCCCTGTGGAACATATTTGGGACAGTTTTTTTGATGATG
CTCAACTCACCGTTTCAGATGATTTTGAGCGTGGTGAACAAATTGCAGCGGAGCGTGAGAGTTTTTGA

Structures

Toxin

• AlphaFold DB: S1PLF5