Detailed information of TA system TA183993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5766567..5767198
• Replicon: chromosome
• Accession: NZ_CP066123
• Organism: Pseudomonas sp. MPFS

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I9018_RS26360
• Protein ID: WP_241107910.1
• Coordinates: 5766567..5766965 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G7A756
• Locus tag: I9018_RS26365
• Protein ID: WP_068588739.1
• Coordinates: 5766965..5767198 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9018_RS26335 (I9018_26250)	5761630..5761995	+	366	WP_241107907.1	hypothetical protein	-
I9018_RS26340 (I9018_26255)	5762004..5762585	-	582	WP_068588876.1	hypothetical protein	-
I9018_RS26345 (I9018_26260)	5762746..5764791	-	2046	WP_241107908.1	c-type cytochrome	-
I9018_RS26350 (I9018_26265)	5765007..5765672	-	666	WP_068588881.1	metalloregulator ArsR/SmtB family transcription factor	-
I9018_RS26355 (I9018_26270)	5765781..5766284	+	504	WP_241107909.1	DUF2938 domain-containing protein	-
I9018_RS26360 (I9018_26275)	5766567..5766965	-	399	WP_241107910.1	tRNA(fMet)-specific endonuclease VapC	Toxin
I9018_RS26365 (I9018_26280)	5766965..5767198	-	234	WP_068588739.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
I9018_RS26370 (I9018_26285)	5767375..5767956	+	582	WP_241110201.1	DedA family protein	-
I9018_RS26375 (I9018_26290)	5768077..5769324	+	1248	WP_241107911.1	aspartate aminotransferase family protein	-
I9018_RS26380 (I9018_26295)	5769350..5769934	-	585	WP_068588731.1	hypothetical protein	-
I9018_RS26385 (I9018_26300)	5769939..5770382	-	444	WP_068588728.1	DUF3592 domain-containing protein	-
I9018_RS26390 (I9018_26305)	5770530..5771360	-	831	WP_241107912.1	amidohydrolase family protein	-
I9018_RS26395 (I9018_26310)	5771521..5771916	+	396	WP_241107913.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14966.22 Da
• Isoelectric Point: 8.4893

>T183993 WP_241107910.1 NZ_CP066123:c5766965-5766567 [Pseudomonas sp. MPFS]
MFKYMLDTNICIFTIKNKPQQVREAFSRNQGRLCISSITLMELIYGAEKSAVPEKNLGRVESFVARLQVLDYDDNAAIHS
GQLQAELAQKGRRIGPYDQLIAGHARSLGLTLVTNNLREFERVPGLRVEDWV

Nucleotide

• Length: 399 bp

>T183993 NZ_CP087290:1385845-1386222 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8732.69 Da
• Isoelectric Point: 4.2092

>AT183993 WP_068588739.1 NZ_CP066123:c5767198-5766965 [Pseudomonas sp. MPFS]
METGSVFKSNRSQAIRMPKAVALPDDVTRVDIVAIGRTRIITPAGESWDSWFDEQDSVSADFMTEREQPAEQVREEF

Nucleotide

• Length: 234 bp

>AT183993 NZ_CP087290:1385424-1385798 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A6G7A756