Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3383505..3384067 Replicon chromosome
Accession NZ_CP066085
Organism Escherichia fergusonii strain FDAARGOS_1032

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag I6H89_RS16175 Protein ID WP_000605676.1
Coordinates 3383789..3384067 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag I6H89_RS16170 Protein ID WP_000781370.1
Coordinates 3383505..3383789 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H89_RS16155 3378864..3381911 + 3048 WP_015953395.1 formate dehydrogenase-N subunit alpha -
I6H89_RS16160 3381924..3382808 + 885 WP_001240580.1 formate dehydrogenase N subunit beta -
I6H89_RS16165 3382801..3383454 + 654 WP_000045645.1 formate dehydrogenase-N subunit gamma -
I6H89_RS16170 3383505..3383789 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
I6H89_RS16175 3383789..3384067 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6H89_RS16180 3384253..3385263 - 1011 WP_000642397.1 alcohol dehydrogenase AdhP -
I6H89_RS16185 3385397..3387094 - 1698 WP_000433464.1 malate dehydrogenase -
I6H89_RS16190 3387251..3387388 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
I6H89_RS16195 3387490..3387705 - 216 WP_000495766.1 biofilm-dependent modulation protein -
I6H89_RS16200 3388048..3388479 + 432 WP_000152299.1 peroxiredoxin OsmC -
I6H89_RS16205 3388540..3388680 + 141 WP_109043809.1 omptin family outer membrane protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T183898 WP_000605676.1 NZ_CP066085:c3384067-3383789 [Escherichia fergusonii]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T183898 NZ_CP087203:5475028-5475306 [Pseudomonas sp. B21-056]
ATGATTAAATCCTTCCAGCACAAAGGTCTTCGCATTTTCTATGAAACGGGTTCGACTCGTGGCATCCGGGCCGACCATGG
AAAGCGCCTGGCTCGCATGCTTCAGTTCATGGACAGGGCGACAGTACCGAACGACCTTGATGTTCCGGGATGGCGTCTGC
ACCCACTCAGGGGAGAACTGGCTGAATACTGGTCCCTGAGCGTTTCGGGAAACTGGCGAATCATTTTTCGTTTTATCGGA
TCGGATATCGAACTGGTCGATTATCTGGATTACCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT183898 WP_000781370.1 NZ_CP066085:c3383789-3383505 [Escherichia fergusonii]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT183898 NZ_CP087203:5475321-5475608 [Pseudomonas sp. B21-056]
ATGCCCATGCACAACCCGCCACACCCTGGCGAAACGCTCCTGATGGATGTGCTGCCCGAACTGGGTATCACCGTCACCGA
ACTGGCGCGGCACCTGGGCTTCGCCCGGCCTCATCTCTCGCGAGTACTGCATGGCCATGCGCCTGTCAGCCCGGATCTGG
CTGTTCGCCTGGAACGCGCAGGAATCGGCAAGGCCAGAGTCTGGTTGGGGGTCCAGACCGACTATGATTTGTGGCAAGCC
GAACAGCGCGAACAACCTCTTATCGAGCCGTTCCTACAACACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References