Detailed information of TA system TA183817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 22739..23286
• Replicon: plasmid unnamed2
• Accession: NZ_CP066034
• Organism: Escherichia coli strain FDAARGOS_1059

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: I6I16_RS26630
• Protein ID: WP_000969996.1
• Coordinates: 23005..23286 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A2W8GN83
• Locus tag: I6I16_RS26625
• Protein ID: WP_000079940.1
• Coordinates: 22739..23008 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6I16_RS26595	17962..18129	-	168	Protein_18	conjugal transfer protein TraC	-
I6I16_RS26600	18383..18916	-	534	WP_001303319.1	transcription termination factor NusG	-
I6I16_RS26605	19358..19645	-	288	WP_000074849.1	conjugal transfer protein TraA	-
I6I16_RS26610	19563..19826	-	264	WP_001324596.1	ash family protein	-
I6I16_RS26615	20799..21830	+	1032	WP_000907875.1	replication initiation protein	-
I6I16_RS26620	22526..22684	+	159	WP_000841008.1	hypothetical protein	-
I6I16_RS26625	22739..23008	+	270	WP_000079940.1	hypothetical protein	Antitoxin
I6I16_RS26630	23005..23286	+	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6I16_RS26635	23486..23752	-	267	WP_000627828.1	hypothetical protein	-
I6I16_RS26640	23752..24246	-	495	WP_021561690.1	hypothetical protein	-
I6I16_RS26645	24302..24904	-	603	WP_000517686.1	hypothetical protein	-
I6I16_RS26650	25013..25180	+	168	Protein_29	hypothetical protein	-
I6I16_RS26655	25211..25546	-	336	WP_001080730.1	colicin 1A immunity protein	-
I6I16_RS26660	25675..26022	+	348	WP_000142456.1	hypothetical protein	-
I6I16_RS26665	26040..26630	-	591	WP_000198612.1	hypothetical protein	-
I6I16_RS26670	26630..26887	-	258	WP_000343101.1	hypothetical protein	-
I6I16_RS26675	27457..27870	+	414	WP_000465046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..88626	88626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T183817 WP_000969996.1 NZ_CP066034:23005-23286 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T183817 NZ_CP087187:258841-259023 [Pseudomonas atacamensis]
ATGCGCAGTCGGGAAATGATCAGGATGATCGAGGACGATGGTTGGTACCTGGTCGCGGTAAAAGGCAGTCACCACCAGTA
CAAGCATTCGTTCAAGCAGGGAAGGGTGACAATCAAGCATCCTGATTCGGATCTGCCCAAGGGTACGATCAACAGCATTT
TGAAACAGGCGGGTTTGAAATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10274.60 Da
• Isoelectric Point: 7.2057

>AT183817 WP_000079940.1 NZ_CP066034:22739-23008 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDMLSHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT183817 NZ_CP087187:259083-259484 [Pseudomonas atacamensis]
ATGAAATTCCCGGTCGTTCTGCACAAGGATGCCGACTCGGACTACGGCGTGATCATTCCGGACGTACCGGGGTGTTATTC
CGCAGGCGCCACGGTAGCCGAAGCTTTCGAAAACACGCAGGAAGCGCTGGCGCTGCACTACGAGGGGCTGGTCGCCGATG
GCGCGCCACTGCCGCAAGTGCGCGACATTGATGCTCATCTTGATAACCCGGATTACGCGGGCGGTATCTGGGGCGTGGTC
GACTTTGACATCACGCCTTACTTCGGCAAATCAGTGCGCTTCAATGCCACATTGCCCGAGCAGTTGCTGGAACGTATTGA
CCAGACCGTCCGGCGAGACCGGCGCTACCGTTCCCGGTCCGGATTTCTGGCGGCCGCTGCGCTGCGCGAATTGTCGGTAT
GA

Structures

Toxin

• AlphaFold DB: E9LLH3

Antitoxin

• AlphaFold DB: A0A2W8GN83