Detailed information of TA system TA183430

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4065323..4066029
• Replicon: chromosome
• Accession: NZ_CP065825
• Organism: Klebsiella pneumoniae strain KpPF25

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: I8N74_RS19960
• Protein ID: WP_024622903.1
• Coordinates: 4065661..4066029 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A485WAM6
• Locus tag: I8N74_RS19955
• Protein ID: WP_023302278.1
• Coordinates: 4065323..4065640 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N74_RS19910	4060464..4061288	+	825	WP_023302271.1	DUF945 domain-containing protein	-
I8N74_RS19915	4061497..4062207	+	711	WP_023302272.1	DeoR family transcriptional regulator	-
I8N74_RS19920	4062233..4062769	+	537	WP_023302273.1	DUF4339 domain-containing protein	-
I8N74_RS19925	4062811..4063248	+	438	WP_023301392.1	hypothetical protein	-
I8N74_RS19930	4063315..4063725	+	411	WP_102047358.1	hypothetical protein	-
I8N74_RS19935	4063803..4064039	+	237	WP_032410024.1	DUF905 domain-containing protein	-
I8N74_RS19940	4064126..4064584	+	459	WP_023302275.1	antirestriction protein	-
I8N74_RS19945	4064593..4065075	+	483	WP_023302276.1	RadC family protein	-
I8N74_RS19950	4065084..4065305	+	222	WP_023302277.1	DUF987 domain-containing protein	-
I8N74_RS19955	4065323..4065640	+	318	WP_023302278.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I8N74_RS19960	4065661..4066029	+	369	WP_024622903.1	TA system toxin CbtA family protein	Toxin
I8N74_RS19965	4066026..4066367	+	342	WP_024622904.1	hypothetical protein	-
I8N74_RS19970	4066490..4069135	-	2646	WP_192869837.1	helix-turn-helix transcriptional regulator	-
I8N74_RS19975	4069509..4070468	+	960	WP_023302280.1	thiamine pyrophosphate-dependent dehydrogenase E1 component subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.82 Da
• Isoelectric Point: 7.2897

>T183430 WP_024622903.1 NZ_CP065825:4065661-4066029 [Klebsiella pneumoniae]
MKNLPATISQAAKPCLSPVAVWQMLLTHLLEQHYGLMLSDTPFSDEAVIQEHIDAGITLANAVNFLVEKYELVRIDRCGF
SSQVQAPYLTATDILHARKACGLMSRYSYREVSNIVLSRSRI

Nucleotide

• Length: 369 bp

>T183430 NZ_CP086997:209675-210001 [Pseudosulfitobacter pseudonitzschiae]
GTGAGGCGTGGTGAGCTGGTGACGGTCGCCCTTCAGGGCGACCACGGCAAGCCGCGTCCCGCGCTTGTGATCCAATCCGA
CCGGTTTCCGGACACCGCCACCACGACCGTCCTTCTGGTTACATCCACCTTGGTGGATGCGCCGTTGATTCGGCTGACGG
TCGAACCCTCTGCGGAGAACGGGTTGCGTATGCGATCACAGATCATGATCGACAAAGCCGTGACAGTTCGAACGGACAAG
CTGGGGAAAGCTTTCGGTCAGCTGGACGATACCGCCATGATCGAGGTGAACCGGGCACTAGCACTGTTTTTAGGAGTGGC
GGGGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11860.67 Da
• Isoelectric Point: 6.4629

>AT183430 WP_023302278.1 NZ_CP065825:4065323-4065640 [Klebsiella pneumoniae]
MSNPTWGLLRDITPCLGARLVQEGNRLYYLVDRASITGKFSDAEILKLDVVFPHFISQMESMLTIGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYLTQR

Nucleotide

• Length: 318 bp

>AT183430 NZ_CP086997:209460-209678 [Pseudosulfitobacter pseudonitzschiae]
ATGCCAACCTCTGTTTCTCACCGTGTCCAGAAGCGTCGCGATGCCTTGCGTGCCGCTGGTTTGCGCCCCGTCCAGATTTG
GGTGCCGGATACCCGCCGCCCCGGTTTCGAGGCTGAGTGCCGCCGCCAGGCTGCAATTGTAGCCAAGGCCGATCGCGCAG
ATGATACTCTGAACGATTTCCTGGATGCCTCCCTGGTCGATGTGGATGACTGGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A485WAM6