Detailed information of TA system TA183321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 3709170..3709743
• Replicon: chromosome
• Accession: NZ_CP065750
• Organism: Pseudomonas stutzeri strain FDAARGOS_875

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W5IUR0
• Locus tag: I6G32_RS17245
• Protein ID: WP_009618209.1
• Coordinates: 3709456..3709743 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W5ISI7
• Locus tag: I6G32_RS17240
• Protein ID: WP_009618210.1
• Coordinates: 3709170..3709469 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G32_RS17210	3704272..3704490	+	219	WP_011829925.1	AlpA family transcriptional regulator	-
I6G32_RS17215	3704531..3705397	+	867	WP_013982110.1	ParA family protein	-
I6G32_RS17220	3705381..3705617	+	237	WP_013982111.1	type II toxin-antitoxin system HicA family toxin	-
I6G32_RS17225	3705610..3707268	+	1659	WP_013982112.1	ParB family protein	-
I6G32_RS17230	3707286..3707846	+	561	WP_013982113.1	DUF2857 domain-containing protein	-
I6G32_RS17235	3707849..3709042	+	1194	WP_013982114.1	hypothetical protein	-
I6G32_RS17240	3709170..3709469	+	300	WP_009618210.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
I6G32_RS17245	3709456..3709743	+	288	WP_009618209.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6G32_RS17250	3709772..3709963	+	192	WP_011805449.1	hypothetical protein	-
I6G32_RS17255	3710087..3710866	+	780	WP_013982115.1	TIGR03761 family integrating conjugative element protein	-
I6G32_RS17260	3710863..3711411	+	549	WP_041771667.1	DUF3158 family protein	-
I6G32_RS17265	3711408..3711791	+	384	WP_004362284.1	single-stranded DNA-binding protein	-
I6G32_RS17270	3712086..3714101	+	2016	WP_013982117.1	DNA topoisomerase III	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3701294..3714516	13222

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-58)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10854.46 Da
• Isoelectric Point: 8.4628

>T183321 WP_009618209.1 NZ_CP065750:3709456-3709743 [Pseudomonas stutzeri]
VRVLEWREAARADLLAIVDYISDDNPDAAQRLKDDIEAKAAKLPERPKLYRPGRVAGTREMVVRANYVVVYMEDTRAVSI
LRVLHAAQQWPPARE

Nucleotide

• Length: 288 bp

>T183321 NZ_CP086891:26109-26504 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCGTTGAGTTCGCGGACACGAATGTCGTTCTTTACCTGCTCGACGACGGCCCAAAGGCCGATCGCGCCGAGGTCAT
CCTGGGGCAGGGGCCCCGGATCAGCGTTCAGGTTCTAAACGAGTCACTGGTGAACTGCCGCCGCAAAGCTGGCCTCGGTT
GGGAGGAAGCAGCGGCCTTTCTCGAAGGCGTGCGCGCCTTGTGTCCCGTCGAAGATCTCACCGTGCAGACCCATGACGTC
GGCCGCGCTTTGGCGGAACGCTACGGCTTCTCGATCTACGACGCGATGATCGTGGCCAGCGCTTTGGTTGCGGGGTGTAC
CACGCTGTGGAGCGAGGACATGCAAGATGGCCTGCTGGTGGAAGGCCAGCTTCGCATCGTCAACCCCTTTGCATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10816.46 Da
• Isoelectric Point: 8.6579

>AT183321 WP_009618210.1 NZ_CP065750:3709170-3709469 [Pseudomonas stutzeri]
MAAQTSMLHIRVDDKLKADAAEKLAGVGLTVSDAVRILLTRVVKEGALPAGLTADPEAYDAWFRAKVQEALADTRPPVPH
QQVMDEAQALIDRKRRART

Nucleotide

• Length: 300 bp

>AT183321 NZ_CP086891:25892-26119 [Pseudosulfitobacter pseudonitzschiae]
ATGCAGGTCGCAAAATGGGGTAACTCGCTGGCCGTCCGTTTGCCCGCGGAGCTCGTCCGAGAGCTTGGTCTCAAGGAGGG
TGATCAGATCGACCTGGTCAAGGACGACGGTCGGGTCAGAGTCCGTCGCCTTGCTCGTGCGGATGAGGTACTTACCGGCC
TGCGCCGCTTCCGGGGCAAGTTGTCCGCAGCAGAACGTCTGAGCCGCGACGACGCACATGAGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A5R9QF16

Antitoxin

• AlphaFold DB: A0A5R9QE11