Detailed information of TA system TA183028

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 503749..504580
• Replicon: chromosome
• Accession: NZ_CP065615
• Organism: Escherichia coli strain FDAARGOS_943

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: I6H00_RS02655
• Protein ID: WP_000854814.1
• Coordinates: 503749..504123 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: I6H00_RS02660
• Protein ID: WP_001285585.1
• Coordinates: 504212..504580 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H00_RS02615	498804..499277	+	474	WP_197939877.1	DNA gyrase inhibitor SbmC	-
I6H00_RS02620	499475..500533	+	1059	WP_001200891.1	FUSC family protein	-
I6H00_RS02625	500705..501034	+	330	WP_000450409.1	DUF496 family protein	-
I6H00_RS02630	501135..501317	-	183	WP_001016348.1	hypothetical protein	-
I6H00_RS02635	501389..501517	+	129	Protein_521	transposase domain-containing protein	-
I6H00_RS02640	501916..503043	-	1128	WP_001063816.1	IS110-like element ISSso6 family transposase	-
I6H00_RS02645	503432..503545	-	114	WP_001161660.1	DUF957 domain-containing protein	-
I6H00_RS02650	503558..503752	-	195	WP_000988600.1	hypothetical protein	-
I6H00_RS02655	503749..504123	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
I6H00_RS02660	504212..504580	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
I6H00_RS02665	504654..504875	-	222	WP_000692323.1	DUF987 domain-containing protein	-
I6H00_RS02670	504938..505414	-	477	WP_001186773.1	RadC family protein	-
I6H00_RS02675	505430..505909	-	480	WP_000860076.1	antirestriction protein	-
I6H00_RS02680	505991..506812	-	822	WP_001234530.1	DUF945 domain-containing protein	-
I6H00_RS02690	508369..508779	-	411	WP_000846713.1	hypothetical protein	-
I6H00_RS02695	508795..509478	-	684	WP_000775500.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	501916..508058	6142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T183028 WP_000854814.1 NZ_CP065615:c504123-503749 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T183028 NZ_CP086626:1082896-1083303 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT183028 WP_001285585.1 NZ_CP065615:c504580-504212 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT183028 NZ_CP086626:1082649-1082915 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60