Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 108944..109701 Replicon plasmid unnamed2
Accession NZ_CP065612
Organism Escherichia coli strain FDAARGOS_945

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag I6H02_RS27510 Protein ID WP_000501969.1
Coordinates 109216..109701 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag I6H02_RS27505 Protein ID WP_011114751.1
Coordinates 108944..109228 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H02_RS27480 104214..104381 + 168 Protein_127 IS66 family insertion sequence element accessory protein TnpB -
I6H02_RS27485 104527..105546 + 1020 WP_038341671.1 IS110-like element ISEc11 family transposase -
I6H02_RS27490 105821..106003 + 183 WP_089519923.1 IS66 family insertion sequence element accessory protein TnpB -
I6H02_RS27495 106023..107624 + 1602 WP_004967149.1 IS66-like element ISSfl3 family transposase -
I6H02_RS27500 107878..108042 - 165 WP_001346193.1 hypothetical protein -
I6H02_RS27505 108944..109228 + 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
I6H02_RS27510 109216..109701 + 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
I6H02_RS27515 109874..110206 - 333 Protein_134 IS4/IS5 family transposase -
I6H02_RS27520 110269..111412 + 1144 Protein_135 IS3 family transposase -
I6H02_RS27525 111451..111591 - 141 Protein_136 IS3 family transposase -
I6H02_RS27530 111661..112848 - 1188 WP_181876988.1 hypothetical protein -
I6H02_RS27535 112918..114126 - 1209 WP_115264394.1 IS91 family transposase -
I6H02_RS27540 114107..114379 - 273 Protein_139 hypothetical protein -
I6H02_RS27545 114546..114665 - 120 Protein_140 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospD3/senA / ospC1 / faeI / faeH / faeF / faeE / faeD / faeC / senB / ospE2 / ospE1 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC4 / ospB / icsP/sopA / nleE / ospC2 1..277227 277227
- inside IScluster/Tn - ospC3 / ospD3/senA / ospC1 97605..120077 22472


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T183003 WP_000501969.1 NZ_CP065612:109216-109701 [Escherichia coli]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T183003 NZ_CP086625:1084430-1084837 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT183003 WP_011114751.1 NZ_CP065612:108944-109228 [Escherichia coli]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT183003 NZ_CP086625:1084183-1084449 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References