Detailed information of TA system TA182826

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25542..25806
• Replicon: plasmid pPinheadLarry_01
• Accession: NZ_CP065468
• Organism: Klebsiella quasipneumoniae strain Pinhead Larry

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: I5M56_RS24825
• Protein ID: WP_001387489.1
• Coordinates: 25654..25806 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	25542..25602 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M56_RS24800	21009..22154	-	1146	WP_000976514.1	class C beta-lactamase CMY-2	-
I5M56_RS24805	22478..22813	-	336	Protein_24	transposase	-
I5M56_RS24810	22872..23840	-	969	WP_020219382.1	IS5 family transposase	-
I5M56_RS24815	23874..24806	-	933	Protein_26	IS1380-like element ISEc9 family transposase	-
I5M56_RS24820	24992..25339	-	348	Protein_27	protein finQ	-
-	25542..25602	-	61	NuclAT_0	-	Antitoxin
-	25542..25602	-	61	NuclAT_0	-	Antitoxin
-	25542..25602	-	61	NuclAT_0	-	Antitoxin
-	25542..25602	-	61	NuclAT_0	-	Antitoxin
I5M56_RS24825	25654..25806	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
I5M56_RS24830	25878..26129	-	252	WP_001291964.1	hypothetical protein	-
I5M56_RS24835	26429..26725	+	297	WP_011264046.1	hypothetical protein	-
I5M56_RS24840	26790..26966	-	177	WP_001054897.1	hypothetical protein	-
I5M56_RS24845	27358..27567	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
I5M56_RS24850	27639..28301	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
I5M56_RS24855	28372..30540	-	2169	WP_023518847.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2 / sul2	-	1..76488	76488

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T182826 WP_001387489.1 NZ_CP065468:25654-25806 [Klebsiella quasipneumoniae]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T182826 NZ_CP086606:54133-54513 [Escherichia fergusonii]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT182826 NZ_CP065468:c25602-25542 [Klebsiella quasipneumoniae]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin