Detailed information of TA system TA182787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 169883..170619
• Replicon: plasmid pGordon_02
• Accession: NZ_CP065462
• Organism: Klebsiella pneumoniae strain Gordon

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: I5M58_RS29020
• Protein ID: WP_003026803.1
• Coordinates: 170137..170619 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I5M58_RS29015
• Protein ID: WP_003026799.1
• Coordinates: 169883..170149 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M58_RS28970	165945..166307	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
I5M58_RS28975	166357..166707	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
I5M58_RS28980	167065..167334	+	270	WP_004152102.1	hypothetical protein	-
I5M58_RS28985	167322..167897	+	576	WP_004152103.1	hypothetical protein	-
I5M58_RS28990	167928..168422	+	495	WP_004152104.1	DNA-binding protein	-
I5M58_RS28995	168466..168834	+	369	WP_004152105.1	hypothetical protein	-
I5M58_RS29000	168868..169071	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
I5M58_RS29005	169120..169377	+	258	WP_004152107.1	hypothetical protein	-
I5M58_RS29010	169453..169707	+	255	WP_004152108.1	hypothetical protein	-
I5M58_RS29015	169883..170149	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I5M58_RS29020	170137..170619	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
I5M58_RS29025	170827..172173	+	1347	WP_077253535.1	ISNCY family transposase	-
I5M58_RS29030	172222..172617	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
I5M58_RS29035	172765..174019	-	1255	Protein_184	IS3 family transposase	-
I5M58_RS29040	174107..175069	-	963	WP_004152113.1	M48 family metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / dfrA14 / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15	-	1..195964	195964

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T182787 WP_003026803.1 NZ_CP065462:170137-170619 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T182787 NZ_CP086572:2733131-2733493 [Staphylococcus argenteus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTTGT
CATTATTCAAAATGACACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCCA
AAATACCGACCCATGTAGAGATTGAAAAGAAAAAATATAAACTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGG
ACACTTGATAAAAAACGTTTAAAAGAAAAACTGACGTACTTATCAGACAACAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAACACAATGGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT182787 WP_003026799.1 NZ_CP065462:169883-170149 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT182787 NZ_CP086572:2732964-2733134 [Staphylococcus argenteus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCAATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66