Detailed information of TA system TA182763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 147881..148791
• Replicon: plasmid pMagicconchshell_02
• Accession: NZ_CP065459
• Organism: Klebsiella pneumoniae strain Magic conch shell

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: I5M59_RS26145
• Protein ID: WP_032413375.1
• Coordinates: 147881..148351 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: I5M59_RS26150
• Protein ID: WP_032413443.1
• Coordinates: 148348..148791 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M59_RS26095	143402..143542	+	141	Protein_144	galactoside O-acetyltransferase	-
I5M59_RS26100	143557..144254	-	698	Protein_145	IS1 family transposase	-
I5M59_RS26105	144347..144712	+	366	Protein_146	Tn3 family transposase	-
I5M59_RS26110	144746..145030	-	285	Protein_147	EAL domain-containing protein	-
I5M59_RS26115	145031..145201	+	171	WP_071571074.1	hypothetical protein	-
I5M59_RS26120	145151..145459	-	309	Protein_149	DDE-type integrase/transposase/recombinase	-
I5M59_RS26125	145476..145637	-	162	Protein_150	IS110 family transposase	-
I5M59_RS26130	145693..146661	-	969	WP_075209834.1	IS5-like element IS903B family transposase	-
I5M59_RS26135	146694..146939	+	246	Protein_152	IS481 family transposase	-
I5M59_RS26140	147511..147771	+	261	WP_194443260.1	hypothetical protein	-
I5M59_RS26145	147881..148351	-	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
I5M59_RS26150	148348..148791	-	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
I5M59_RS26155	148891..149403	-	513	Protein_156	DUF4113 domain-containing protein	-
I5M59_RS26160	149504..149689	-	186	WP_071571073.1	DUF2188 domain-containing protein	-
I5M59_RS26165	149709..150007	+	299	Protein_158	transposase	-
I5M59_RS26170	150208..150435	-	228	Protein_159	transposase	-
I5M59_RS26175	150493..150840	-	348	WP_032413377.1	hypothetical protein	-
I5M59_RS26180	150977..152155	-	1179	WP_032413379.1	cystathionine gamma-synthase family protein	-
I5M59_RS26185	152202..153200	-	999	WP_032413381.1	N(2)-acetyl-L-2,4-diaminobutanoate deacetylase DoeB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..204071	204071
	-	inside	IScluster/Tn	-	-	143557..150465	6908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T182763 WP_032413375.1 NZ_CP065459:c148351-147881 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T182763 NZ_CP086564:2672039-2672182 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT182763 WP_032413443.1 NZ_CP065459:c148791-148348 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT182763 NZ_CP086564:c2672376-2672114 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTACA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATATCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTTTTCTTTTACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4