Detailed information of TA system TA182762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 60528..61264
• Replicon: plasmid pMagicconchshell_02
• Accession: NZ_CP065459
• Organism: Klebsiella pneumoniae strain Magic conch shell

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: I5M59_RS25655
• Protein ID: WP_003026803.1
• Coordinates: 60528..61010 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I5M59_RS25660
• Protein ID: WP_003026799.1
• Coordinates: 60998..61264 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M59_RS25625	56325..56828	+	504	Protein_50	GGDEF domain-containing protein	-
I5M59_RS25630	56920..57033	-	114	WP_077252861.1	IS3 family transposase	-
I5M59_RS25635	57158..57328	+	171	Protein_52	LysR family transcriptional regulator	-
I5M59_RS25640	57453..57569	-	117	Protein_53	transposase	-
I5M59_RS25645	58510..59286	-	777	Protein_54	DUF1173 family protein	-
I5M59_RS25650	59319..60287	+	969	WP_194443255.1	IS5-like element IS903B family transposase	-
I5M59_RS25655	60528..61010	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
I5M59_RS25660	60998..61264	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I5M59_RS25665	61709..61939	-	231	WP_004206660.1	hypothetical protein	-
I5M59_RS25670	61953..62156	-	204	WP_004213596.1	hemolysin expression modulator Hha	-
I5M59_RS25675	62217..62708	-	492	WP_004206662.1	hypothetical protein	-
I5M59_RS25680	62842..63039	-	198	Protein_61	hypothetical protein	-
I5M59_RS25685	63130..64098	+	969	WP_013362812.1	IS5-like element IS903B family transposase	-
I5M59_RS25690	64150..64341	-	192	Protein_63	IS1 family transposase	-
I5M59_RS25695	64397..64600	+	204	Protein_64	LysR family transcriptional regulator	-
I5M59_RS25700	64669..65577	-	909	WP_032413424.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..204071	204071
	-	inside	IScluster/Tn	-	-	53396..64098	10702

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T182762 WP_003026803.1 NZ_CP065459:c61010-60528 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T182762 NZ_CP086564:c2666755-2666414 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT182762 WP_003026799.1 NZ_CP065459:c61264-60998 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT182762 NZ_CP086564:c2666985-2666755 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66