Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4932884..4933509 Replicon chromosome
Accession NZ_CP065457
Organism Klebsiella pneumoniae strain Magic conch shell

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I5M59_RS24005 Protein ID WP_002882817.1
Coordinates 4933126..4933509 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I5M59_RS24000 Protein ID WP_004150355.1
Coordinates 4932884..4933126 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M59_RS23975 4928875..4929474 + 600 WP_194443214.1 glucose-1-phosphatase -
I5M59_RS23980 4929468..4930328 + 861 WP_004146232.1 virulence factor BrkB family protein -
I5M59_RS23985 4930325..4930762 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I5M59_RS23990 4930807..4931748 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I5M59_RS23995 4931762..4932679 - 918 WP_023302328.1 alpha/beta hydrolase -
I5M59_RS24000 4932884..4933126 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I5M59_RS24005 4933126..4933509 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I5M59_RS24010 4933683..4934612 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I5M59_RS24015 4934609..4935244 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I5M59_RS24020 4935241..4936143 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182761 WP_002882817.1 NZ_CP065457:4933126-4933509 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T182761 NZ_CP086564:c2593274-2593131 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACGGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182761 WP_004150355.1 NZ_CP065457:4932884-4933126 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182761 NZ_CP086564:2593011-2593198 [Enterococcus faecalis]
TGCGCTATAATGAAAATGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACTTTTT
GAATTATTTAAAAATAACCGTACTCGGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGT
GCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References