Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2325487..2327075 Replicon chromosome
Accession NZ_CP065457
Organism Klebsiella pneumoniae strain Magic conch shell

Toxin (Protein)


Gene name hipA Uniprot ID A0A486KW17
Locus tag I5M59_RS11235 Protein ID WP_004190166.1
Coordinates 2325753..2327075 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag I5M59_RS11230 Protein ID WP_002904790.1
Coordinates 2325487..2325753 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M59_RS11205 2320638..2321561 + 924 WP_004190172.1 LysR family hpxDE operon transcriptional regulator HpxR -
I5M59_RS11210 2321542..2322696 - 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
I5M59_RS11215 2323042..2324421 + 1380 WP_004184021.1 purine permease -
I5M59_RS11220 2324399..2324899 + 501 WP_194443094.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
I5M59_RS11225 2324896..2325222 + 327 WP_004143476.1 hydroxyisourate hydrolase -
I5M59_RS11230 2325487..2325753 + 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
I5M59_RS11235 2325753..2327075 + 1323 WP_004190166.1 type II toxin-antitoxin system HipA family toxin Toxin
I5M59_RS11240 2327092..2328384 - 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
I5M59_RS11245 2328526..2329530 - 1005 WP_004190163.1 type 1 fimbrial protein -
I5M59_RS11250 2329518..2332019 - 2502 WP_004190161.1 fimbrial biogenesis outer membrane usher protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49171.46 Da        Isoelectric Point: 7.5357

>T182748 WP_004190166.1 NZ_CP065457:2325753-2327075 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTTLLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T182748 NZ_CP086560:32046-32189 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT182748 WP_002904790.1 NZ_CP065457:2325487-2325753 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT182748 NZ_CP086560:c32383-32121 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTATA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486KW17

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References