Detailed information of TA system TA182702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 136704..137361
• Replicon: plasmid pSeaSuperman_02
• Accession: NZ_CP065446
• Organism: Klebsiella pneumoniae strain Sea Superman

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: I5M62_RS28585
• Protein ID: WP_000270043.1
• Coordinates: 136704..137054 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5M62_RS28590
• Protein ID: WP_000124640.1
• Coordinates: 137059..137361 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M62_RS28550	133178..133675	-	498	WP_000062185.1	hypothetical protein	-
I5M62_RS28555	133678..134166	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
I5M62_RS28560	134263..134598	+	336	WP_000683476.1	hypothetical protein	-
I5M62_RS28565	134613..135083	-	471	WP_001281821.1	hypothetical protein	-
I5M62_RS28570	135076..135447	-	372	WP_000516916.1	hypothetical protein	-
I5M62_RS28575	135458..135652	-	195	WP_000343597.1	hypothetical protein	-
I5M62_RS28580	135993..136541	-	549	WP_001061574.1	transcriptional regulator	-
I5M62_RS28585	136704..137054	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5M62_RS28590	137059..137361	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
I5M62_RS28595	137388..137681	-	294	WP_001239997.1	chromosome segregation protein ParM	-
I5M62_RS28600	137769..138041	-	273	WP_001043047.1	HU family DNA-binding protein	-
I5M62_RS28605	138099..138626	-	528	WP_001236377.1	thermonuclease family protein	-
I5M62_RS28610	138857..139645	-	789	WP_194315300.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / aac(3)-IId / rmtB	-	1..139649	139649

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T182702 WP_000270043.1 NZ_CP065446:136704-137054 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T182702 NZ_CP086544:1042991-1043326 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT182702 WP_000124640.1 NZ_CP065446:137059-137361 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT182702 NZ_CP086544:1042743-1042991 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9