Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 9428..10167 Replicon plasmid pDirtyDan_01
Accession NZ_CP065437
Organism Klebsiella pneumoniae strain Dirty Dan

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag I6Z21_RS25535 Protein ID WP_013087172.1
Coordinates 9682..10167 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag I6Z21_RS25530 Protein ID WP_012540086.1
Coordinates 9428..9694 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6Z21_RS25500 4660..5196 - 537 WP_012540238.1 GNAT family N-acetyltransferase -
I6Z21_RS25505 5321..7072 - 1752 WP_162866166.1 arsenical pump-driving ATPase -
I6Z21_RS25510 7099..7461 - 363 WP_094716534.1 arsenic metallochaperone ArsD family protein -
I6Z21_RS25515 7537..8082 - 546 WP_012540227.1 sigma-70 family RNA polymerase sigma factor -
I6Z21_RS25520 8091..8804 - 714 WP_012540184.1 arsenical resistance protein ArsH -
I6Z21_RS25525 8806..9129 - 324 WP_012540250.1 metalloregulator ArsR/SmtB family transcription factor -
I6Z21_RS25530 9428..9694 + 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
I6Z21_RS25535 9682..10167 + 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
I6Z21_RS25540 10507..10668 + 162 WP_004118124.1 type I toxin-antitoxin system Hok family toxin -
I6Z21_RS25545 11363..11635 + 273 WP_004182067.1 hypothetical protein -
I6Z21_RS25550 11632..11982 + 351 WP_023320095.1 hypothetical protein -
I6Z21_RS25555 12618..12974 + 357 WP_004152717.1 hypothetical protein -
I6Z21_RS25560 13035..13247 + 213 WP_004152718.1 hypothetical protein -
I6Z21_RS25565 13258..13482 + 225 WP_004152719.1 hypothetical protein -
I6Z21_RS25570 13563..13883 + 321 WP_004152720.1 type II toxin-antitoxin system RelE/ParE family toxin -
I6Z21_RS25575 13873..14151 + 279 WP_004152721.1 helix-turn-helix domain-containing protein -
I6Z21_RS25580 14152..14565 + 414 WP_004182074.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaDHA-1 / qnrB4 / aph(6)-Id / blaTEM-1B / aac(3)-IId / floR / sul2 / aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / tet(A) - 1..147528 147528


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T182634 WP_013087172.1 NZ_CP065437:9682-10167 [Klebsiella pneumoniae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T182634 NZ_CP086520:1042991-1043326 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT182634 WP_012540086.1 NZ_CP065437:9428-9694 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT182634 NZ_CP086520:1042743-1042991 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References