Detailed information of TA system TA182549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 639367..641117
• Replicon: chromosome
• Accession: NZ_CP065404
• Organism: Paraburkholderia caribensis strain IAC-BECa88

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: I5286_RS19280
• Protein ID: WP_227245711.1
• Coordinates: 639367..640260 (+)
• Length: 298 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: I5286_RS19285
• Protein ID: WP_107202304.1
• Coordinates: 640257..641117 (+)
• Length: 287 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5286_RS19250	634729..635801	-	1073	Protein_567	porin	-
I5286_RS19255	636242..636559	+	318	WP_035998998.1	non-heme iron oxygenase ferredoxin subunit	-
I5286_RS19260	636561..636872	+	312	WP_035999001.1	hypothetical protein	-
I5286_RS19265	636927..638015	+	1089	WP_062917262.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
I5286_RS19270	638008..638766	+	759	WP_227245710.1	SDR family oxidoreductase	-
I5286_RS19275	638802..639341	+	540	WP_035999007.1	cupin domain-containing protein	-
I5286_RS19280	639367..640260	+	894	WP_227245711.1	alpha/beta fold hydrolase	Toxin
I5286_RS19285	640257..641117	+	861	WP_107202304.1	IclR family transcriptional regulator	Antitoxin
I5286_RS19290	641117..641923	+	807	WP_062917259.1	SDR family oxidoreductase	-
I5286_RS19295	641916..643574	+	1659	WP_227245713.1	thiamine pyrophosphate-binding protein	-
I5286_RS19300	643567..644397	+	831	WP_054933316.1	aspartate dehydrogenase	-
I5286_RS19305	644422..645918	+	1497	WP_227245714.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (50-276)

Antitoxin

• Transcription regulator IclR, N-terminal (26-74)

Sequences

Toxin

Protein

• Length: 298 a.a.
• Molecular weight: 31116.16 Da
• Isoelectric Point: 6.2320

>T182549 WP_227245711.1 NZ_CP065404:639367-640260 [Paraburkholderia caribensis]
MSAALPVTVDRTVTLETRLSHFPARRIAIALQCAIGYREAGAGHADALPLVLLHGIGSGAASWVQQLEALGTTRRVFAWD
APGYGESTPVASASPKADEYAGALDAWLDALAVERCVLVGHSLGAIIAGSFAASRAQRVAGLLLISPAAGYGTASEDVRA
SKRDSRLAMLAELGPQGLADQRSANMLSPYADEASRDWVRWNMSRIVPTGYAQATHLLANADLASDVARVDGPIAVAVGA
EDSITPPSSCERIAQAARTRLQIIPRAGHAGYIEAANEYTALIDAFCRASDGQWSKA

Nucleotide

• Length: 894 bp

>T182549 NZ_CP086490:c2542205-2542029 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 287 a.a.
• Molecular weight: 31407.84 Da
• Isoelectric Point: 6.5092

>AT182549 WP_107202304.1 NZ_CP065404:640257-641117 [Paraburkholderia caribensis]
MTPELTGTAPEAEDDRNDAGYRVPGLERGLRILTEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADRDRN
YRLGVAVLRLGFEYLSSLELTDLGLPLIEALRDATGLTTHIVIRDGRDVVFVAKAQSHAPVFSSVKVNVGTRLPAHATTH
GHVLMGDLSLDELRALYPEKTLERFTKHTPATVDELYERVKDDAVRGFAISESSFENGISVISAPVRNDTGRIAAVITTT
IPRPQIDAALLDGGLIDKVRGAAEQLSLRLNYRPSAESRYMKALGL

Nucleotide

• Length: 861 bp

>AT182549 NZ_CP086490:c2541983-2541567 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA