Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/PIN-RelB
Location 1370231..1371111 Replicon chromosome
Accession NZ_CP065397
Organism Bifidobacterium longum strain I2-2-3

Toxin (Protein)


Gene name relE Uniprot ID F8APN2
Locus tag I3K78_RS05895 Protein ID WP_007052625.1
Coordinates 1370231..1370722 (-) Length 164 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A4R0V8T5
Locus tag I3K78_RS05900 Protein ID WP_013410773.1
Coordinates 1370719..1371111 (-) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I3K78_RS05875 (I3K78_05865) 1365284..1366546 + 1263 WP_223616533.1 histidine kinase -
I3K78_RS05880 (I3K78_05870) 1366581..1367306 + 726 WP_007056206.1 response regulator transcription factor -
I3K78_RS05885 (I3K78_05875) 1367346..1367987 + 642 WP_007052627.1 GNAT family N-acetyltransferase -
I3K78_RS05890 (I3K78_05880) 1368076..1370181 + 2106 WP_007052626.1 DUF262 domain-containing protein -
I3K78_RS05895 (I3K78_05885) 1370231..1370722 - 492 WP_007052625.1 type II toxin-antitoxin system VapC family toxin Toxin
I3K78_RS05900 (I3K78_05890) 1370719..1371111 - 393 WP_013410773.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
I3K78_RS05905 (I3K78_05895) 1371257..1373956 - 2700 WP_007054466.1 aconitate hydratase AcnA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-146)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(6-53)


Sequences

Toxin        


Download         Length: 164 a.a.        Molecular weight: 18531.70 Da        Isoelectric Point: 6.2348

>T182523 WP_007052625.1 NZ_CP065397:c1370722-1370231 [Bifidobacterium longum]
MRQSKQVSILCDTNILIYYMLCTPRFIKDVMKLFERCVEREVKLSCASLSLKDVFYITPERFKERFMNDESMVVATTAKL
ILPEVAWSGISKLKELMTIVPIGAEECELAQSLRHAHNDFEDDLIIAAAQQSGATYVVTYDKKLAKHFPEYCKTPAEIVA
ALA

Download         Length: 492 bp

>T182523 NZ_CP086480:3792687-3792965 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 15205.33 Da        Isoelectric Point: 5.4043

>AT182523 WP_013410773.1 NZ_CP065397:c1371111-1370719 [Bifidobacterium longum]
MTMVQMNVRIDAKLKNEVEEVLKGKGVSVSDVIRSLWLYIADRKEVPALETTAEEQAREEEKQRKLKLIREGAGYVHKEL
VKAGLISEDTDLMEGLTYKQFRDEIADERLKEYRHQSDYARYLEKERSLS

Download         Length: 393 bp

>AT182523 NZ_CP086480:3792424-3792684 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F8APN2

Antitoxin

Source ID Structure
AlphaFold DB A0A4R0V8T5

References