Detailed information of TA system TA182514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1243217..1243860
• Replicon: chromosome
• Accession: NZ_CP065386
• Organism: Glaesserella parasuis strain LHDR_HPS_1_2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMB1
• Locus tag: I5Z97_RS06035
• Protein ID: WP_005714197.1
• Coordinates: 1243516..1243860 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5Z97_RS06030
• Protein ID: WP_016527809.1
• Coordinates: 1243217..1243513 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5Z97_RS06005	1238368..1239207	-	840	WP_075630451.1	NAD-dependent deacetylase	-
I5Z97_RS06010	1239234..1240070	-	837	WP_075630450.1	NAD-dependent deacetylase	-
I5Z97_RS06015	1240147..1240899	-	753	WP_080652560.1	NAD-dependent protein deacylase	-
I5Z97_RS06020	1240868..1241611	-	744	WP_021112448.1	protein phosphatase 2C domain-containing protein	-
I5Z97_RS06025	1242192..1243154	-	963	WP_021112452.1	WYL domain-containing protein	-
I5Z97_RS06030	1243217..1243513	-	297	WP_016527809.1	helix-turn-helix domain-containing protein	Antitoxin
I5Z97_RS06035	1243516..1243860	-	345	WP_005714197.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5Z97_RS06040	1244075..1246054	+	1980	WP_075630449.1	exoribonuclease II	-
I5Z97_RS06045	1246164..1247255	+	1092	WP_005714201.1	murein transglycosylase A	-
I5Z97_RS06050	1247438..1248034	-	597	WP_021110885.1	DedA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1226141..1246054	19913

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (52-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13263.22 Da
• Isoelectric Point: 5.9233

>T182514 WP_005714197.1 NZ_CP065386:c1243860-1243516 [Glaesserella parasuis]
METEEYLTFIETKVFEEDRKALMSDDEYQKFQAYLLESHELGDFIQNTGGCQKIRWKLESNNKGKSGGVRVIYYVVTKQG
KLLLMMMYAKSKQDNMSDKQKAMLKAVVSQLSEE

Nucleotide

• Length: 345 bp

>T182514 NZ_CP086480:1907831-1908085 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11196.11 Da
• Isoelectric Point: 8.8246

>AT182514 WP_016527809.1 NZ_CP065386:c1243513-1243217 [Glaesserella parasuis]
MEKELFERLMESAREMVAIEKGEIEVPPHRIHIHKIPNVKAIRTQHQMKQAEFASLLGVSQNLVQSWEIGRRTPNGAARK
LLAIIEQQPELIPVLQGL

Nucleotide

• Length: 297 bp

>AT182514 NZ_CP086480:1907583-1907834 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: U4SMB1