Detailed information of TA system TA182269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1043752..1044395
• Replicon: chromosome
• Accession: NZ_CP065178
• Organism: Pectobacterium quasiaquaticum strain A398-S21-F17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IG605_RS04860
• Protein ID: WP_116167083.1
• Coordinates: 1043752..1044096 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IG605_RS04865
• Protein ID: WP_116167084.1
• Coordinates: 1044096..1044395 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IG605_RS04835	1039110..1041122	-	2013	WP_250116857.1	phage tail protein	-
IG605_RS04840 (IG605_004830)	1041119..1041730	-	612	WP_193407843.1	phage tail protein I	-
IG605_RS04845 (IG605_004835)	1041723..1042631	-	909	WP_193407842.1	baseplate assembly protein	-
IG605_RS04850 (IG605_004840)	1042635..1042979	-	345	WP_103184661.1	GPW/gp25 family protein	-
IG605_RS04855 (IG605_004845)	1042976..1043611	-	636	WP_193407851.1	phage baseplate assembly protein V	-
IG605_RS04860 (IG605_004850)	1043752..1044096	+	345	WP_116167083.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IG605_RS04865 (IG605_004855)	1044096..1044395	+	300	WP_116167084.1	XRE family transcriptional regulator	Antitoxin
IG605_RS04870 (IG605_004860)	1044431..1044880	-	450	WP_116167085.1	phage virion morphogenesis protein	-
IG605_RS04875 (IG605_004865)	1044873..1045331	-	459	WP_193407841.1	phage tail protein	-
IG605_RS04880 (IG605_004870)	1045325..1045537	-	213	WP_116167087.1	hypothetical protein	-
IG605_RS04885	1045572..1045841	-	270	WP_226373406.1	Rz1-like lysis system protein LysC	-
IG605_RS04890 (IG605_004875)	1045762..1046124	-	363	Protein_935	Rz-like lysis system protein LysB	-
IG605_RS04895 (IG605_004880)	1046124..1046612	-	489	WP_039491306.1	glycoside hydrolase family 104 protein	-
IG605_RS04900 (IG605_004885)	1046599..1046892	-	294	WP_039350700.1	phage holin family protein	-
IG605_RS04905 (IG605_004890)	1046896..1047099	-	204	WP_193407840.1	tail protein X	-
IG605_RS04910 (IG605_004895)	1047099..1047602	-	504	WP_193407839.1	head completion/stabilization protein	-
IG605_RS04915 (IG605_004900)	1047694..1048353	-	660	WP_161529625.1	terminase endonuclease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1032009..1064838	32829

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13406.24 Da
• Isoelectric Point: 6.8726

>T182269 WP_116167083.1 NZ_CP065178:1043752-1044096 [Pectobacterium quasiaquaticum]
MWTVVLTERFTAWLEEQEGSLQEKMAADLMRLEFYGPQLPRPYADGVKGSRYRNMKELRVQHAGHPIRAFFAFDPERQAI
VLCAGDKSNDKRFYDTMIRIADDEFTEHLAKRNH

Nucleotide

• Length: 345 bp

>T182269 NZ_CP086372:c1491602-1491468 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10971.78 Da
• Isoelectric Point: 5.8651

>AT182269 WP_116167084.1 NZ_CP065178:1044096-1044395 [Pectobacterium quasiaquaticum]
MKTLKDFIAAQSPESQARIKEMADEMILETGLQMLREELQLSQKTVADAMGIKQPAITQIEQRGNDVKLATLKRYIEAMG
GKLSLTVELPDGGGRVFHI

Nucleotide

• Length: 300 bp

>AT182269 NZ_CP086372:c1491818-1491666 [Xanthomonas oryzae pv. oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA