Detailed information of TA system TA181841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1084521..1085148
• Replicon: chromosome
• Accession: NZ_CP064855
• Organism: Erwinia amylovora strain Ea1/79Sm

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D2T444
• Locus tag: IYV53_RS05000
• Protein ID: WP_004156337.1
• Coordinates: 1084521..1084739 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: IYV53_RS05005
• Protein ID: WP_013035913.1
• Coordinates: 1084759..1085148 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IYV53_RS04970	1080507..1080845	+	339	WP_004156319.1	P-II family nitrogen regulator	-
IYV53_RS04975	1080863..1082164	+	1302	WP_004172214.1	ammonium transporter AmtB	-
IYV53_RS04980	1082239..1083099	-	861	WP_004156324.1	acyl-CoA thioesterase II	-
IYV53_RS04985	1083324..1083881	+	558	WP_004156333.1	YbaY family lipoprotein	-
IYV53_RS04990	1083904..1084221	-	318	WP_004156334.1	MGMT family protein	-
IYV53_RS05000	1084521..1084739	-	219	WP_004156337.1	hemolysin expression modulator Hha	Toxin
IYV53_RS05005	1084759..1085148	-	390	WP_013035913.1	Hha toxicity modulator TomB	Antitoxin
IYV53_RS05010	1085295..1085648	-	354	WP_004156342.1	hypothetical protein	-
IYV53_RS05015	1086420..1086566	-	147	WP_004156349.1	type B 50S ribosomal protein L36	-
IYV53_RS05020	1086554..1086835	-	282	WP_004156350.1	type B 50S ribosomal protein L31	-
IYV53_RS05025	1086983..1090129	-	3147	WP_004156353.1	multidrug efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8627.06 Da
• Isoelectric Point: 8.9007

>T181841 WP_004156337.1 NZ_CP064855:c1084739-1084521 [Erwinia amylovora]
MTDKLLTKTDYLMRLRRCRSIDTLERVIEKNKYELSDDELAVFYSAADHRLAELTMNKLYDKVPVAVWKFVR

Nucleotide

• Length: 219 bp

>T181841 NZ_CP086184:1569125-1569376 [Xanthomonas oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 14965.70 Da
• Isoelectric Point: 4.7040

>AT181841 WP_013035913.1 NZ_CP064855:c1085148-1084759 [Erwinia amylovora]
MDEYSPKRHDIAQLKFLCENLYDESLATLGDSHHGWVNDPTSTSNLQLNDLIEHIAAFTMNYKIKHIEDSDLISQIDEYL
DDTFMLFSNYGVNNLDLQRWQKSAKRLFNIFAKECVMSQIQSSHSFSSP

Nucleotide

• Length: 390 bp

>AT181841 NZ_CP086184:1568820-1569098 [Xanthomonas oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Structures

Toxin

• AlphaFold DB: A0A831EPQ0