Detailed information of TA system TA181639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1853110..1853774
• Replicon: chromosome
• Accession: NZ_CP064692
• Organism: Bradyrhizobium sp. 191

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A151FTR6
• Locus tag: IVB23_RS08535
• Protein ID: WP_061878073.1
• Coordinates: 1853367..1853774 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: IVB23_RS08530
• Protein ID: WP_247803215.1
• Coordinates: 1853110..1853370 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IVB23_RS08520 (IVB23_08490)	1849933..1850859	+	927	WP_061878070.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
IVB23_RS08525 (IVB23_08495)	1850896..1852056	+	1161	WP_061878071.1	adenosyl-hopene transferase HpnH	-
IVB23_RS08530 (IVB23_08500)	1853110..1853370	+	261	WP_247803215.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IVB23_RS08535 (IVB23_08505)	1853367..1853774	+	408	WP_061878073.1	type II toxin-antitoxin system VapC family toxin	Toxin
IVB23_RS08540 (IVB23_08510)	1853894..1854286	-	393	WP_247803216.1	type II toxin-antitoxin system VapC family toxin	-
IVB23_RS08545 (IVB23_08515)	1854273..1854548	-	276	WP_061878645.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
IVB23_RS08550 (IVB23_08520)	1854738..1855487	-	750	WP_247803217.1	phosphorylase	-
IVB23_RS08555 (IVB23_08525)	1855484..1857436	-	1953	WP_247803218.1	squalene--hopene cyclase	-
IVB23_RS08560 (IVB23_08530)	1857502..1858770	-	1269	WP_247803219.1	hydroxysqualene dehydroxylase HpnE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (8-45)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15106.24 Da
• Isoelectric Point: 4.7783

>T181639 WP_061878073.1 NZ_CP064692:1853367-1853774 [Bradyrhizobium sp. 191]
MTKWLLDTNAVIALVTRRSEPLLRRVESSEPGALAISAIVTHELYFGAYRSQKVEFNLETLRLLFADLEILDLDQEDARA
TGEIRADLARRGTPIGPYDLLIAGQAIARGLPLVSNNTAEFQRIAGLRLEDWTRD

Nucleotide

• Length: 408 bp

>T181639 NZ_CP085987:c4345599-4345186 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9703.90 Da
• Isoelectric Point: 10.4368

>AT181639 WP_247803215.1 NZ_CP064692:1853110-1853370 [Bradyrhizobium sp. 191]
MSHIAKVFKSGNSQAVRLPKDFRFSVDEVEVSREGEAVILRPRRSATGPWTSLRAAVARGFSPDFMAEGREQPEEQTRPD
LNQAFR

Nucleotide

• Length: 261 bp

>AT181639 NZ_CP085987:c4345846-4345580 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A151FTR6