Detailed information of TA system TA181259

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3756643..3757172
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: IRY56_RS17430
• Protein ID: WP_003417760.1
• Coordinates: 3756915..3757172 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: IRY56_RS17425
• Protein ID: WP_003417757.1
• Coordinates: 3756643..3756918 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS17400	3751674..3752972	-	1299	WP_011799343.1	PPE family protein	-
IRY56_RS17405	3753441..3754878	-	1438	Protein_3437	FAD-binding oxidoreductase	-
IRY56_RS17410	3754981..3755370	+	390	WP_010950876.1	DUF732 domain-containing protein	-
IRY56_RS17415	3755384..3755677	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
IRY56_RS17420	3755674..3756519	-	846	WP_010950877.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
IRY56_RS17425	3756643..3756918	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
IRY56_RS17430	3756915..3757172	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
IRY56_RS17435	3757214..3758404	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
IRY56_RS17440	3758521..3758889	+	369	WP_003417765.1	FHA domain-containing protein	-
IRY56_RS17445	3758886..3759437	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
IRY56_RS17450	3759444..3760025	-	582	WP_003417769.1	ATP/GTP-binding protein	-
IRY56_RS17455	3760006..3760374	-	369	WP_003417772.1	DUF742 domain-containing protein	-
IRY56_RS17460	3760352..3760744	-	393	WP_011799344.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T181259 WP_003417760.1 NZ_CP064405:3756915-3757172 [Mycobacterium tuberculosis variant bovis BCG]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T181259 NZ_CP085815:4350573-4350971 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCGGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAATTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT181259 WP_003417757.1 NZ_CP064405:3756643-3756918 [Mycobacterium tuberculosis variant bovis BCG]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT181259 NZ_CP085815:4350346-4350573 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGAGGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTTTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94