Detailed information of TA system TA181258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3694513..3695187
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: IRY56_RS17225
• Protein ID: WP_003417282.1
• Coordinates: 3694513..3694941 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: IRY56_RS17230
• Protein ID: WP_003417286.1
• Coordinates: 3694945..3695187 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS17200	3690335..3690736	-	402	WP_003417264.1	cytidine deaminase	-
IRY56_RS17205	3690877..3691311	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
IRY56_RS17210	3691308..3691742	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
IRY56_RS17215	3691871..3693643	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
IRY56_RS17220	3693643..3694434	+	792	WP_010950870.1	succinate dehydrogenase iron-sulfur subunit	-
IRY56_RS17225	3694513..3694941	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
IRY56_RS17230	3694945..3695187	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
IRY56_RS17235	3695309..3695923	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
IRY56_RS17240	3695920..3696585	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
IRY56_RS17245	3696586..3697119	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
IRY56_RS17250	3697116..3697490	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
IRY56_RS17255	3697587..3698651	-	1065	WP_003913037.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T181258 WP_003417282.1 NZ_CP064405:c3694941-3694513 [Mycobacterium tuberculosis variant bovis BCG]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T181258 NZ_CP085815:4339669-4340082 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT181258 WP_003417286.1 NZ_CP064405:c3695187-3694945 [Mycobacterium tuberculosis variant bovis BCG]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT181258 NZ_CP085815:4339422-4339688 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGACGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59