Detailed information of TA system TA181252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3056688..3057258
• Replicon: chromosome
• Accession: NZ_CP064405
• Organism: Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: IRY56_RS14300
• Protein ID: WP_003414166.1
• Coordinates: 3056688..3057044 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: IRY56_RS14305
• Protein ID: WP_003901465.1
• Coordinates: 3057028..3057258 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IRY56_RS14280	3052140..3053828	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
IRY56_RS14285	3053832..3054158	-	327	WP_003414157.1	hypothetical protein	-
IRY56_RS14290	3054331..3054918	+	588	WP_003914429.1	DUF3558 family protein	-
IRY56_RS14295	3054937..3056586	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
IRY56_RS14300	3056688..3057044	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
IRY56_RS14305	3057028..3057258	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
IRY56_RS14310	3057301..3058344	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
IRY56_RS14315	3058535..3058810	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
IRY56_RS14320	3058986..3059240	-	255	WP_003917684.1	hypothetical protein	-
IRY56_RS14325	3059388..3059792	+	405	WP_003414181.1	hypothetical protein	-
IRY56_RS14330	3059789..3059980	+	192	WP_003414184.1	hypothetical protein	-
IRY56_RS14335	3060044..3061333	+	1290	Protein_2828	transposase family protein	-
IRY56_RS14340	3061567..3061824	+	258	WP_003899489.1	hypothetical protein	-
IRY56_RS14345	3061929..3062240	+	312	WP_010950777.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T181252 WP_003414166.1 NZ_CP064405:c3057044-3056688 [Mycobacterium tuberculosis variant bovis BCG]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T181252 NZ_CP085815:c2907254-2906970 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT181252 WP_003901465.1 NZ_CP064405:c3057258-3057028 [Mycobacterium tuberculosis variant bovis BCG]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT181252 NZ_CP085815:c2907486-2907244 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3