Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3056688..3057258 Replicon chromosome
Accession NZ_CP064405
Organism Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag IRY56_RS14300 Protein ID WP_003414166.1
Coordinates 3056688..3057044 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag IRY56_RS14305 Protein ID WP_003901465.1
Coordinates 3057028..3057258 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRY56_RS14280 3052140..3053828 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
IRY56_RS14285 3053832..3054158 - 327 WP_003414157.1 hypothetical protein -
IRY56_RS14290 3054331..3054918 + 588 WP_003914429.1 DUF3558 family protein -
IRY56_RS14295 3054937..3056586 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
IRY56_RS14300 3056688..3057044 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
IRY56_RS14305 3057028..3057258 - 231 WP_003901465.1 antitoxin MazE Antitoxin
IRY56_RS14310 3057301..3058344 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
IRY56_RS14315 3058535..3058810 + 276 WP_003911993.1 DUF1778 domain-containing protein -
IRY56_RS14320 3058986..3059240 - 255 WP_003917684.1 hypothetical protein -
IRY56_RS14325 3059388..3059792 + 405 WP_003414181.1 hypothetical protein -
IRY56_RS14330 3059789..3059980 + 192 WP_003414184.1 hypothetical protein -
IRY56_RS14335 3060044..3061333 + 1290 Protein_2828 transposase family protein -
IRY56_RS14340 3061567..3061824 + 258 WP_003899489.1 hypothetical protein -
IRY56_RS14345 3061929..3062240 + 312 WP_010950777.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T181252 WP_003414166.1 NZ_CP064405:c3057044-3056688 [Mycobacterium tuberculosis variant bovis BCG]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T181252 NZ_CP085815:c2907254-2906970 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT181252 WP_003901465.1 NZ_CP064405:c3057258-3057028 [Mycobacterium tuberculosis variant bovis BCG]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT181252 NZ_CP085815:c2907486-2907244 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References