Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2827252..2827889 Replicon chromosome
Accession NZ_CP064405
Organism Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag IRY56_RS13060 Protein ID WP_003413180.1
Coordinates 2827252..2827647 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag IRY56_RS13065 Protein ID WP_003413183.1
Coordinates 2827644..2827889 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRY56_RS13020 2823337..2823996 + 660 WP_003900846.1 LppA family lipoprotein -
IRY56_RS13025 2823993..2824652 + 660 WP_003900847.1 hypothetical protein -
IRY56_RS13030 2824649..2825311 + 663 WP_003900848.1 hypothetical protein -
IRY56_RS13035 2825308..2825586 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
IRY56_RS13040 2825679..2826092 + 414 WP_003413164.1 PIN domain nuclease -
IRY56_RS13045 2826131..2826388 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
IRY56_RS13050 2826385..2826762 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
IRY56_RS13055 2826778..2827152 - 375 WP_003413177.1 hypothetical protein -
IRY56_RS13060 2827252..2827647 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
IRY56_RS13065 2827644..2827889 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
IRY56_RS13070 2828300..2828719 - 420 WP_003413190.1 A24 family peptidase -
IRY56_RS13075 2828731..2829558 - 828 WP_010950736.1 shikimate dehydrogenase -
IRY56_RS13080 2829536..2830789 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
IRY56_RS13085 2830782..2831294 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T181248 WP_003413180.1 NZ_CP064405:c2827647-2827252 [Mycobacterium tuberculosis variant bovis BCG]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T181248 NZ_CP085815:2246416-2246634 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT181248 WP_003413183.1 NZ_CP064405:c2827889-2827644 [Mycobacterium tuberculosis variant bovis BCG]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT181248 NZ_CP085815:2246014-2246388 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References