Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1684535..1685151 Replicon chromosome
Accession NZ_CP064405
Organism Mycobacterium tuberculosis variant bovis BCG strain BCG SL 222 Sofia

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag IRY56_RS07820 Protein ID WP_003407593.1
Coordinates 1684834..1685151 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7U4BU54
Locus tag IRY56_RS07815 Protein ID WP_011799188.1
Coordinates 1684535..1684837 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IRY56_RS07805 1680421..1682268 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
IRY56_RS07810 1682269..1684521 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
IRY56_RS07815 1684535..1684837 + 303 WP_011799188.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
IRY56_RS07820 1684834..1685151 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
IRY56_RS07825 1685148..1686152 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
IRY56_RS07830 1686205..1687494 + 1290 WP_003407599.1 serine hydrolase -
IRY56_RS07835 1687567..1688340 - 774 WP_003912632.1 class I SAM-dependent methyltransferase -
IRY56_RS07840 1688398..1688589 - 192 WP_003407609.1 dodecin family protein -
IRY56_RS07845 1688620..1688844 - 225 WP_160522526.1 NUDIX domain-containing protein -
IRY56_RS07850 1689114..1690142 + 1029 WP_011799189.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T181229 WP_003407593.1 NZ_CP064405:1684834-1685151 [Mycobacterium tuberculosis variant bovis BCG]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T181229 NZ_CP085813:4606769-4607044 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11199.50 Da        Isoelectric Point: 9.5564

>AT181229 WP_011799188.1 NZ_CP064405:1684535-1684837 [Mycobacterium tuberculosis variant bovis BCG]
VPFLVALSGIILGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT181229 NZ_CP085813:4606506-4606766 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCACGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BU54

References