Detailed information of TA system TA181199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5281298..5281884
• Replicon: chromosome
• Accession: NZ_CP064403
• Organism: Pseudomonas aeruginosa strain WTJH12

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: ITG71_RS24525
• Protein ID: WP_003120987.1
• Coordinates: 5281585..5281884 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ITG71_RS24520
• Protein ID: WP_003448662.1
• Coordinates: 5281298..5281588 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG71_RS24500 (ITG71_24485)	5276449..5276658	+	210	WP_003105733.1	cold-shock protein	-
ITG71_RS24505 (ITG71_24490)	5276880..5278769	+	1890	WP_016851610.1	hypothetical protein	-
ITG71_RS24510 (ITG71_24495)	5278766..5280742	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
ITG71_RS24515 (ITG71_24500)	5280883..5281227	+	345	WP_016851612.1	hypothetical protein	-
ITG71_RS24520 (ITG71_24505)	5281298..5281588	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
ITG71_RS24525 (ITG71_24510)	5281585..5281884	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG71_RS24530 (ITG71_24515)	5282086..5283210	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
ITG71_RS24535 (ITG71_24520)	5283210..5284919	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
ITG71_RS24540 (ITG71_24525)	5284923..5286248	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
ITG71_RS24545 (ITG71_24530)	5286238..5286771	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5254319..5340689	86370

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T181199 WP_003120987.1 NZ_CP064403:c5281884-5281585 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T181199 NZ_CP085811:663499-663807 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGGTTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT181199 WP_003448662.1 NZ_CP064403:c5281588-5281298 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT181199 NZ_CP085811:663257-663496 [Salmonella enterica subsp. enterica serovar Schwarzengrund]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCTGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTGCTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: G8CP73