Detailed information of TA system TA181167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5858000..5858595
• Replicon: chromosome
• Accession: NZ_CP064395
• Organism: Pseudomonas aeruginosa strain SRRSH1408

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: ITG72_RS27250
• Protein ID: WP_003113526.1
• Coordinates: 5858317..5858595 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ITG72_RS27245
• Protein ID: WP_003099268.1
• Coordinates: 5858000..5858305 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITG72_RS27210 (ITG72_27210)	5853151..5853999	+	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
ITG72_RS27220 (ITG72_27220)	5854166..5855107	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
ITG72_RS27225 (ITG72_27225)	5855224..5855838	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
ITG72_RS27230 (ITG72_27230)	5855880..5856464	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
ITG72_RS27235 (ITG72_27235)	5856505..5857605	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
ITG72_RS27245 (ITG72_27245)	5858000..5858305	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
ITG72_RS27250 (ITG72_27250)	5858317..5858595	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ITG72_RS32485	5858648..5858776	-	129	Protein_5403	integrase	-
ITG72_RS27255 (ITG72_27255)	5858924..5861152	+	2229	WP_003099265.1	TonB-dependent receptor	-
ITG72_RS27260 (ITG72_27260)	5861222..5861869	-	648	WP_003095021.1	carbonate dehydratase	-
ITG72_RS27265 (ITG72_27265)	5861931..5863169	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T181167 WP_003113526.1 NZ_CP064395:c5858595-5858317 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T181167 NZ_CP085808:c3565877-3565593 [Salmonella enterica subsp. enterica serovar Typhi]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT181167 WP_003099268.1 NZ_CP064395:c5858305-5858000 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT181167 NZ_CP085808:c3566109-3565867 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: V6ALY3