Detailed information of TA system TA181031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2707554..2708175
• Replicon: chromosome
• Accession: NZ_CP064378
• Organism: Legionella pneumophila subsp. pneumophila strain PartS-Lpneumophila-RM8376

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q5ZRG7
• Locus tag: IUJ54_RS12055
• Protein ID: WP_010948600.1
• Coordinates: 2707554..2707775 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IUJ54_RS12060
• Protein ID: WP_010948599.1
• Coordinates: 2707885..2708175 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUJ54_RS12035 (IUJ54_12000)	2702716..2703894	-	1179	WP_010948604.1	serine hydrolase domain-containing protein	-
IUJ54_RS12040 (IUJ54_12005)	2704424..2705386	+	963	WP_015443886.1	lytic murein transglycosylase	-
IUJ54_RS12045 (IUJ54_12010)	2705690..2706736	+	1047	WP_010948602.1	FAD-dependent oxidoreductase	-
IUJ54_RS12050 (IUJ54_12015)	2706846..2707349	+	504	WP_015443887.1	hypothetical protein	-
IUJ54_RS12055 (IUJ54_12020)	2707554..2707775	+	222	WP_010948600.1	hypothetical protein	Toxin
IUJ54_RS12060 (IUJ54_12025)	2707885..2708175	+	291	WP_010948599.1	helix-turn-helix domain-containing protein	Antitoxin
IUJ54_RS12065 (IUJ54_12030)	2708464..2708655	-	192	WP_014327021.1	hypothetical protein	-
IUJ54_RS12070 (IUJ54_12035)	2709023..2710465	+	1443	WP_010948598.1	ATP-binding protein	-
IUJ54_RS12075 (IUJ54_12040)	2710627..2712114	-	1488	WP_010948597.1	lpg2912 family Dot/Icm T4SS effector	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-88)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8288.46 Da
• Isoelectric Point: 3.7468

>T181031 WP_010948600.1 NZ_CP064378:2707554-2707775 [Legionella pneumophila subsp. pneumophila]
MIFIETPIFTEDVKELLSDEEYAEFQEYLADNPLAGDVIQQTGKLANWQTAKSSMVISRQMGSEAVLELFIIM

Nucleotide

• Length: 222 bp

>T181031 NZ_CP085799:3747078-3747563 [Salmonella enterica subsp. enterica serovar Enteritidis]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGTGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.68 Da
• Isoelectric Point: 8.0670

>AT181031 WP_010948599.1 NZ_CP064378:2707885-2708175 [Legionella pneumophila subsp. pneumophila]
MDKKLFERLYESMTQMNEIIDGERVPSREFVVDAVHVKEIRKKTGLTQEKFCHLIDVSIGTLRNWEQGRREPTGPAKALL
RAINKDPEHVLAALAG

Nucleotide

• Length: 291 bp

>AT181031 NZ_CP085799:3746803-3747090 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: Q5ZRG7